两种品系小鼠建立登革病毒感染的湿热证模型研究

作者	单位
朱利	南方医科大学公共卫生与热带医学院病毒研究所
赵卫	南方医科大学公共卫生与热带医学院病毒研究所
熊建英	南方医科大学公共卫生与热带医学院病毒研究所
万成松	南方医科大学公共卫生与热带医学院病毒研究所
马丹娟	南方医科大学公共卫生与热带医学院病毒研究所
林培政	广州中医药大学温病教研室
黄仕营	广州中医药大学温病教研室
曹虹	南方医科大学公共卫生与热带医学院病毒研究所

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中文摘要:

目的建立两种不同品系小鼠登革病毒感染的湿热证模型,并比较感染情况差异,确定适合造模的小鼠。方法根据中医温病湿热证的造模方法,采用复合因素:高糖高脂饲料加高温舱加感染因子(登革病毒),分别处理BALB/C和C57BL/6小鼠,同时设正常对照组(对照组)、病毒组(单纯病毒感染造模)、湿热组(单纯湿热条件干预造模),通过观察小鼠的体温、血小板数量、分离血清中病毒、肝脏病理学改变及血清学指标比较模型建立的情况。结果造模后小鼠于高温舱处理时出现低热。与对照组比较,BALB/C模型组血小板降低,两种小鼠模型组和病毒组血清AST均升高,BALB/C模型组、湿热组血清TC、TG升高,差异有统计学意义(P<0.05)。各组肝组织均有不同程度的病理改变,以BALB/C模型组病变最严重。用Real-time PCR检测造模后血清病毒滴度,各组无明显差异,病毒含量2.9×104~5.5×104拷贝数/mL。结论初步构建登革病毒感染的湿热证小鼠模型,比较BALB/C和C57BL/6小鼠感染情况,BALB/C更适合造模。

英文摘要:

Objective To establish two strains of mouse developing dampness-heat syndrome models infected by Dengue virus and to compare the difference in infection,so as to choose a suitable mouse strain for modeling.MethodsAccording to the modeling methods of the seasonal febrile disease of the dampness-heat syndrome in Chinese medicine,BALB/C and C57BL/6 mice were respectively treated with complex factors as the high glucose and high fat forage + high-temperature chamber + Dengue virus.At the same time a normal control group,the virus infection group(modeled by Dengue virus infection),and the dampness-heat group(modeling by pure dampness heat circumstance) were set up.Changes of the body temperature,platelet counts,virus in the separate serum,pathological changes of the liver,and serological indicators were observed to compare the modeling difference.Results After modeling low-grade fever appeared in mice in the high-temperature chamber.Compared with the normal control group,the platelet count decreased in mice of the BALB/C model group.AST increased in both BALB/C and C57BL/6 mice and the virus infection group.TC and TG increased in BALB/C model group and the dampness-heat group,with statistical significance(P<0.05).Various degrees of pathological changes were shown in the liver tissue of each group,with the most severe one in the BALB/C model group.The serum virus titers were detected with Real-time PCR after modeling.The virus load was 2.9×104-5.5×104 copies/mL.No significant difference was found among these groups.Conclusions The mouse model of dampness-heat syndrome infected by Dengue virus was primarily established.When compared the infection between BALB/C mice and C57BL/6 mice,BALB/C mice were more suitable for modeling.

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