中药SPPW方剂对人胃癌细胞侵袭和转移能力的影响

作者	单位
邹百仓	西安交通大学医学院第二附属医院消化科
赵菊辉	西安交通大学医学院第二附属医院消化科
史海涛	西安交通大学医学院第二附属医院消化科
毛艳	西安市中医医院老年病科
董蕾	西安交通大学医学院第二附属医院消化科
党晓燕	西安交通大学医学院第二附属医院消化科

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中文摘要:

目的观察由半枝莲、黄芪、甘草等组成的中药SPPW方剂对人胃癌细胞侵袭和转移能力的影响,阐明中药SPPW抗胃癌转移的作用及可能的机理。方法体外传代培养人胃癌细胞株SGC-7901,待细胞成对数生长期时,随机分为中药高、中、低剂量组、5-氟尿嘧啶(5-FU)组及阴性对照组。药物(分别为SP-PW高、中、低剂量,5-FU,生理盐水)干预胃癌细胞48h后,采用MTT法检测胃癌细胞对matrigel的黏附能力;Transwell小室测定胃癌细胞的侵袭及迁移能力;Western blot和RT-PCR测定基质金属蛋白酶9(MMP-9)和血管内皮生长因子(VEGF)的蛋白及mRNA表达变化。结果与阴性对照组比较,中药各剂量组及5-FU组均可明显抑制胃癌细胞的黏附、侵袭及迁移能力(P<0·05,P<0·01),下调MMP-9和VEGF蛋白及mRNA表达水平(P<0·01)。中药各剂量组呈显著的剂量效应关系(P<0·01)。与5-FU组比较,中药高剂量组在抑制胃癌细胞黏附、迁移能力及降低VEGF蛋白表达和MMP-9、VEGFmRNA表达方面差异均有统计学意义(P<0·01)。结论中药SPPW可降低胃癌细胞对matrigel的黏附能力,降低胃癌细胞的侵袭及迁移能力,同时下调MMP-9及VEGF蛋白及mRNA表达水平,这可能是其影响胃癌细胞侵袭与转移的机制之一。

英文摘要:

Objective To clarify the action and possible mechanisms of SPPW,a Chinese herbal preparation consisting of Herba Scutellariae Barbatae,Radix Astragalus,Radix Glycyrrhizae,etc.,in suppressing the metastasis of human gastric cancer,by way of observing its effect on the invasive and metastatic capacities of gastric cancer cells.Methods In vitro serial sub-cultured human gastric cancer cell line SGC-7901 at the logarithmic growth phase were randomly divided into 5 groups,i.e.the negative control group,the 4 treatment groups intervened respectively with SPPW at three different doses(high,middle,and low),and 5-FU.The adhesion capacities of gastric cancer cells to matrigel were detected by MTT assay 48 h after intervention.The invasive and migratory capacities of gastric cancer cells were determined by Transwell assay.The mRNA and protein expressions of metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF) in gastric cancer cells were detected by Western blot and Real-time polymerase chain reaction(RT-PCR) respectively.Results Compared with the negative control group,the adhesive,invasive and migratory capacities of gastric cancer cells were all significantly inhibited in the four treatment groups(P<0.05,P<0.01).In addition,the protein and mRNA expressions of MMP-9 and VEGF were down-regulated(P<0.01).Significant dose-dependent relation existed in the three SPPW treatment groups(P<0.01).Compared with the 5-FU treatment group,the high dose SPPW treatment group showed significant difference in inhibiting the adhesive and metastatic capacities of gastric cancer cells,lowering VEGF protein expression,and mRNA expressions of MMP-9 and VEGF(P<0.01).Conclusions SPPW could lower the adhesion of gastric cancer cells to matrigel,and lower the invasion and migration of gastric cancer cells.Meanwhile,it could down-regulate the mRNA and protein expressions of MMP-9 and VEGF,which may possibly be one of its mechanisms for influencing the invasion and metastasis of gastric cancer cells.

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