藿香正气提取物对腹泻型肠易激综合征模型大鼠肠动力调控机理研究

目的探讨藿香正气提取物对腹泻型肠易激综合征(D-IBS)模型大鼠肠动力紊乱的调节作用。方法将80只实验大鼠随机分为两部分,一部分用于碳末推进实验,另一部分用于结肠转运实验,每部分40只,各分5组,分别为空白对照组,模型组,中药高、中、低剂量组,每组8只。空白对照组为正常大鼠。模型组及中药各剂量组以25℃番泻叶水煎剂2mL(0·2g/mL)灌胃加四肢束缚应激2h,连续6天,建立D-IBS大鼠模型。中药各剂量组造模后分别灌服804、536、268mg/kg藿香正气提取物。采用碳末推进、结肠转运、硝酸还原酶法、ELISA法及免疫组化法观察藿香正气提取物对D-IBS模型大鼠小肠推进率及血清一氧化氮(NO)、5-羟色胺(5-HT)、结肠黏膜肠嗜铬细胞(EC)含量变化的影响。结果与模型组比较,藿香正气提取物可降低D-IBS模型大鼠小肠推进率,减弱结肠转运效应,提高大鼠血清NO含量,降低5-HT浓度,减少EC数量。结论藿香正气提取物对D-IBS模型大鼠肠道功能紊乱具有调节作用。

英文摘要:

Objective To study the regulatory effect of Huoxiang Zhengqi Extract(HZE) on the intestinal mobility in rat model of diarrhea-predominant irritable bowel syndrome(D-IBS).Methods Eighty experimental rats were randomly divided into two parts,forty in each.Rats in one part were used in the carbon powder experiment,while the rest rats were used in the gastrointestinal transit test.Rats in each part were divided into five groups,i.e.,the blank control group,the model group,and the high,moderate,and low HZE treated groups,eight in each.Normal rats consisted of the blank control group.Except for the blank control group,D-IBS model was established by gastrogavage of senna at the dose of 0.2 g/mL at 25 ℃ with 2-h restraint stress of extremities for six successive days.After modeling,high(804 mg/kg),moderate(536 mg/kg),and low(268 mg/kg) dose HZE was respectively administered to rats of the three corresponding groups.Effect of HZE on the small intestine propulsion rate and serum levels of nitric oxide(NO),5-hydroxytryptamine(5-HT) as well as chromaffin cells in colonic epithelium(EC) were detected by carbon powder experiment,gastrointestinal transit test,nitrate reductase method,ELISA,and immunohistochemistry,respectively.Results Compared with the model group,HZE could lower the small intestine propulsion rate of D-IBS model rats,attenuate the colon transition,improve serum NO level,lower 5-HT level,and lessen the amount of EC.Conclusion HZE showed regulatory effect on the intestinal function of D-IBS rats.

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