头针对脑缺血再灌注大鼠内源性神经干细胞增殖及迁移的影响

作者	单位
邹燃	湖北中医药大学针骨学院
周利	湖北中医药大学针骨学院
张晓艳	湖北中医药大学针骨学院
张红星	武汉市中西医结合医院针灸科
王琼	武汉市中西医结合医院针灸科
刘银妮	湖北中医药大学针骨学院

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中文摘要:

目的研究脑缺血大鼠内源性神经干细胞(neural stemc ells,NSCs)增殖、迁移及头针对其干预情况,探讨头针治疗脑缺血病的作用机制。方法健康Wistar大鼠100只,随机分为假手术组(10只)、模型组(45只)及头针组(45只)。采用改良的线栓法制作成大脑中动脉闭阻(middle cerebral artery occlusion,MCAO)模型;假手术组不插线栓,不做再灌注,余同造模大鼠。造模后模型组与假手术组大鼠行常规饲养,不予任何治疗;头针组于缺血再灌注成功后即行头针治疗,每天1次,连续治疗。各组大鼠分别于造模后7、14、28d后腹腔注射5-溴脱氧尿核苷(bromodeoxyuridine,BrdU,50mg/kg)后麻醉处死。对各组大鼠进行神经功能缺损评分(neurological severity score,NSS),免疫荧光法计数各组大鼠海马区(dentate gyrus,DG)BrdU阳性细胞及BrdU/唾液酸-神经细胞黏附分子(PSA-NCAM)阳性双标细胞。结果与模型组同期比较,头针组各时间点NSS评分降低,其中在28d时差异有统计学意义(P<0·05)。模型组各时间点BrdU及BrdU/PSA-NCAM阳性细胞数较假手术组同期明显增加,头针组各时间点BrdU及BrdU/PSA-NCAM阳性细胞数较模型组同期明显增加,差异均有统计学意义(P<0·01)。结论头针能促进脑缺血再灌注大鼠内源性NSCs增殖和迁移,这可能是头针治疗脑缺血病的机制之一。

英文摘要:

Objective To study the proliferation and migration of neural stem cells(NSCs) of acute cerebral ischemia rats and the intervention of scalp-acupuncture(SA),and to study its action of mechanism in treating cerebral ischemia.Methods One hundred healthy Wistar rats were randomly divided into the sham-operation group(n=10),the model group(n=45),and the SA group(n=45).The middle cerebral artery occlusion(MCAO) model was established using the modified suture method.No suture or perfusion was given to rats in the sham-operation group,but these rats received the same procedures as those for modeled rats.After modeling routine feeding was given to rats in the model group and the sham-operative group without any other treatment.SA was successively given to rats in the SA group after successful ischemia reperfusion,once daily.Rats were anesthetized and sacrificed by peritoneally injecting bromodeoxyuridine(BrdU) at the dose of 50 mg/kg on the 7th(T1),14th day(T2),and 28th day(T3) after modeling.Neurological severity score(NSS) was assessed.The BrdU positive cells and the BrdU/PSA-NCAM positive cells of the dentate gyrus(DG) were counted using immunofluorescence assay.Results Compared with the model group at the same time points,the NSS decreased in the SA group.Significant difference was shown at T3(P<0.05).Compared with the sham-operative group at the same time point,the BrdU positive cells and BrdU/PSA-NCAM positive cells of the model group obviously increased.Compared with the model group at the same time point,the BrdU positive cells and BrdU/PSA-NCAM positive cells of the SA group obviously increased,showing significant difference(P<0.01).Conclusions SA could promote the proliferation and migration of endogenous NSCs,which may possibly be one of its mechanisms in treating cerebral ischemia.

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