益气活血复方联合运动疗法对慢性心力衰竭大鼠心肌组织MMP-1及Ⅲ型胶原表达的影响

Effects of Yiqi Huoxue Compound Combined with Exercise Therapy on MMP-1 and Collagen Type Ⅲ Expressions of Cardiac Muscle in Chronic Heart Failure Rats

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中文关键词: 益气活血复方慢性心力衰竭基质金属蛋白酶-1 Ⅲ型胶原

英文关键词:Yiqi Huoxue Compound chronic heart failure matrix metalloproteinase-1 collagen type Ⅲ

基金项目:辽宁省自然科学基金项目(No.20092036)

作者	单位
张艳	辽宁中医药大学附属医院科研处
马金	辽宁卫生职业技术学院康复技术教研室
礼海	沈阳市第二中医医院医务科

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中文摘要:

目的探讨益气活血复方联合运动疗法对心肌梗死后慢性心力衰竭(chronic heart failure,CHF)大鼠心室重构的影响及其作用机制。方法 60只健康SD大鼠,选取8只为空白对照组,其他大鼠利用冠脉结扎法结合力竭式游泳与减食法制备CHF模型。将造模成功的大鼠随机分为模型组(8只)、中药组(8只)、西药组(8只)、中药加运动组(10只)及运动组(10只)5组。空白对照组和模型组给予常规等容积蒸馏水灌胃,每次3mL;中药组及中药加运动组按每天9·2g生药/kg给予益气活血复方灌胃;西药组按每天1·5mg生药/kg给予赖诺普利灌胃,均每天2次,持续6周。中药加运动组及运动组同时给予运动疗法,每天1次。取大鼠左心室非梗死区心肌组织,实时荧光定量PCR法和免疫组化法检测基质金属蛋白酶-1(MMP-1)和Ⅲ型胶原mRNA及蛋白含量。结果与空白对照组比较,模型组MMP-1 mRNA表达及平均灰度值升高,Ⅲ型胶原mRNA表达及平均灰度值降低,差异均有统计学意义(P<0·05,P<0·01)。与模型组比较,中药组、西药组、中药加运动组MMP-1 mRNA表达及平均灰度值降低(P<0·01),以中药加运动组最低;西药组、中药加运动组Ⅲ型胶原mRNA表达及平均灰度值升高(P<0·01)。结论益气活血复方联合运动疗法能减轻心室重构程度,其机制可能为抑制MMP-1表达,提高Ⅲ型胶原表达,进而减轻甚至逆转心肌纤维化。

英文摘要:

Objective To study the effects and mechanisms of Yiqi Huoxue Compound combined with exercise therapy on the ventricular remodeling of chronic heart failure(CHF) rats after myocardial infarction.Methods Eight rats were selected from sixty healthy SD rats as the blank control group,and the rest was used to prepare CHF rat model by coronary artery ligation in combination with exhausted swimming and food reducing.The successfully modeled rats were randomly divided into five groups,i.e.,the model group(n=8),the Chinese medicine group(n=8),the Western medicine group(n=8),the Chinese medicine+exercise group(n=10),and the exercise group(n=10).Routine gastrogavage of equal volume distilled water was given to rats in the blank control group and the model group respectively,3 mL each time.Yiqi Huoxue Compound was given by gastrogavage to rats in the Chinese medicine group and the Chinese medicine+exercise group at the daily dose of 9.2 g crude drug/kg.Lisinopril was given by gastrogavage to rats in the Western medicine group at the daily dose of 1.5 mg crude drug/kg.The medication was administered twice daily for six successive weeks.The Chinese medicine+exercise group and the exercise group adopted exercise therapy,once daily.The myocardial tissue of rats’ non-infarcted left ventricle was taken out.mRNA and protein contents of matrix metalloproteinase-1(MMP-1) and collagen type Ⅲ were detected by Real-time fluorescent quantitation PCR.Results Compared with the blank control group,the mRNA expression and the average gray value of MMP-1 increased,while those of collagen type Ⅲ decreased in the model group,showing statistical difference(P<0.05,P<0.01).Compared with the model group,the mRNA expression and the average gray value of MMP-1 decreased in the Chinese medicine group,the Western medicine group,and the Chinese medicine+exercise group,showing statistical significance(P<0.01).It was lowest in the Chinese medicine+exercise group.The mRNA expression and the average gray value of collagen type Ⅲ increased in the Western medicine group and the Chinese medicine+exercise group,showing statistical difference(P<0.01).Conclusions Yiqi Huoxue Compound combined wih exercise therapy could attenuate the ventricular remodeling degree.Its mechanism might be to inhibit the MMP-1 expression,improve the expression of collagen type Ⅲ,thus alleviating and even reversing myocardial fibrosis.

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