当归补血汤干预移植肌卫星细胞受体小鼠造血功能重建的研究

作者	单位
赵舒武	天津中医药大学中医学院组织学与胚胎学教研室
王学谦	天津医院
王晓玲	天津中医药大学中医学院组织学与胚胎学教研室
汪雅妮	天津中医药大学中医学院组织学与胚胎学教研室
汪涛	天津中医药大学中医学院组织学与胚胎学教研室

摘要点击次数: 1297

全文下载次数: 2

中文摘要:

目的观察当归补血汤对移植骨骼肌卫星细胞(muscle satellite cell,MSC)受体小鼠造血功能重建的影响。方法分离雄性同系小鼠的MSC并培养鉴定。经8Gy137Cs-γ射线照射的雌性昆明种受体鼠随机分为6组:空白对照组、移植MSC组、当归补血汤不同剂量(1、3、5、10倍临床等效剂量灌胃7天)干预的移植雄性MSC4组。观察移植后1、2、3周受体鼠脾集落形成单位(colony forming unit spleen,CFU-S)数、外周血白细胞(WBC)、血红蛋白(Hb)、血小板(PLT)的变化,3周存活率等指标,并采用PCR方法鉴定其造血重建的来源。结果培养的MSC呈desmin染色阳性;第2周时移植MSC各组WBC明显升高(P<0.05),给药2、3、4组Hb明显回升(P<0.05);第3周时与移植MSC组比较,给药3、4组WBC及Hb升高明显(P<0.05),给药4组PLT恢复显著(P<0.05)。第2周时与空白对照组比较,给药3、4组CFU-S明显增多(P<0.05),对移植的存活小鼠进行Y染色体PCR分析,证实重建雌性受体鼠造血功能的细胞来自雄性供体。结论当归补血汤干预有助于移植MSC的受体鼠造血功能重建。

英文摘要:

Objective To study the effects of Danggui Buxue Decoction on the hematopoietic reconstruction of mice transplanted by muscle satellite cells(MSCs).Methods MSCs were procured from newly born male mice of the homologous series.The female Kunming receptor mice irradiated with 8Gy137Cs-γ ray were randomly divided into six groups,i.e.,the blank control group,the transplanted MSC group,four groups intervened by different doses of Danggui Buxue Decoction after transplanted MSC (gastrogavaged by one,three,five,and ten times of clinically equivalent dose for seven days,as the DGBX 1 group,the DGBX 2 group,the DGBX 3 group,and the DGBX 4 group).After transplantation changes of splenic colony forming unit spleen (CFU-S),white blood cells (WBC),hemoglobin (Hb),and platelet (PLT) in the peripheral blood were observed in pos-transplanted 1-,2-,and 3-week receptor mice.The 3-week survival rate was calculated.The source of hematopoietic reconstruction was identified using PCR.Results The desmin protein was positive in the cultured MSCs.WBC of each transplanted MSC group obviously increased at the 2nd week (P<0.05).Hb obviously increased in the DGBX 2 group,the DGBX 3 group,and the DGBX 4 group (P<0.05).WBC and Hb obviously increased in the DGBX 3 group and the DGBX 4 group when compared with the transplanted MSC group at the 3rd week (P<0.05).The recovery of PLT was significant in the DGBX 4 group (P<0.05).Compared with the blank control group at the 2nd week,CFU-S obviously increased in the DGBX 3 group and the DGBX 4 group (P<0.05).PCR results of Y chromosome in the survived transplant mice indicated that the hematopoietic cells of reconstruction female receptor mice were originated from male donors.Conclusion The hematopoiesis of mice transplanted by muscle satellite cells could be constructed after intervention of Danggui Buxue Decoction.

关闭