通冠胶囊对骨髓单个核细胞向损伤血管内膜迁移分化的影响

作者	单位
张敏州	广州中医药大学第二附属医院ICU
张俭	广州中医药大学第二附属医院ICU
王磊	广州中医药大学第二附属医院ICU
陈鹏	广州中医药大学第二附属医院ICU

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中文摘要:

目的探讨通冠胶囊对骨髓单个核细胞(bone marrow mononuclear cells,BMMNCs)向损伤血管内膜迁移分化的影响。方法建立大鼠颈总动脉损伤模型,从供体大鼠骨髓中分离单个核细胞,经荧光染料PKH26标记后由大鼠尾静脉注射入颈总动脉损伤的大鼠体内,通冠胶囊组给予通冠胶囊混悬液灌胃,盐水对照组给予等量的生理盐水灌胃,4周后取颈总动脉,冰冻切片。观察两组颈动脉内膜面积和血管内膜中膜(intima area/media area,Ai/Am)比值的差异;荧光显微镜下观察两组骨髓单个核细胞向颈动脉内膜迁移的差异;ELISA法检测两组大鼠血清血管内皮生长因子(VEGF)和基质衍生因子-1(SDF-1)水平的差异。结果通冠胶囊组颈动脉横切和纵切面血管内膜有红色荧光表达,而盐水对照组血管切片仅有散在荧光光点;通冠胶囊组血管内膜面积和血管内膜中膜比值均较盐水对照组明显降低(P<0·01);2周、4周后通冠胶囊组VEGF和SDF-1水平均较盐水对照组有明显增强(P<0·01)。结论通冠胶囊能够动员骨髓单个核细胞向损伤血管内膜迁移,并参与损伤血管内膜的修复。

英文摘要:

Objective To observe the effects of Tongguan Capsule on the mobilization and differentiation of bone marrow mononuclear cells(BMMNCs) to the injured carotid arteries.Methods The rat model of injured carotid arteries was established.Mononuclear cells were separated from the bone marrow of donor rats,which were labeled by PKH 26 and then injected through the tail vein to the recipient rats with injured carotid arteries.The rats were randomly divided into two groups.Tongguan Capsule suspension was administered by gastrogavage to rats in the experiment group,while equal volume of normal saline was given to rats in the control group.Four weeks later the injured carotid arteries were harvested and frozen sections were made to observe the differences of intima area/media area ratio(Ai/Am).The difference of BMMNCs migrating to the injured carotid arteries between the two groups was observed under fluorescence microscope.The difference of the vascular endothelial growth factor(VEGF) and stromal derived factor-1(SDF-1) levels in serum were detected by ELISA.Results The PKH 26 labeled cells appeared in the experiment group,while only little scattered fluorescent light points was presented in the control group.The intima area and the ratio of Ai/Am of the experiment group decreased more obviously than that of the control group(P<0.01).Two and 4 weeks later the VEGF and SDF-1 levels in serum of the experiment group were more obviously enhanced than those of the control group(P<0.01).Conclusion Tongguan Capsule could promote the BMMNCs transplanting towards the intima of injured carotid arteries,and take part in the repair of the intima of injured carotid arteries.

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