| 周兴,曾顺,毛武塬,卢芳国,刘朝圣,林奕涛,何清湖.知柏地黄汤对解脲支原体感染大鼠生精细胞凋亡及Caspase-3、Caspase-9表达的影响[J].中国中西医结合杂志,2011,31(9):1254-1258 |
| 知柏地黄汤对解脲支原体感染大鼠生精细胞凋亡及Caspase-3、Caspase-9表达的影响 |
| Effects of Zhibai Dihuang Decotion on UU-infected Rat’s Spermatogenic Cell Apoptosis and Expressions of Caspase-3 and Caspase-9 |
| 免费下载全文 查看/发表评论 下载PDF阅读器 |
| |
| DOI： |
| 中文关键词: 知柏地黄汤 解脲支原体 生精细胞 线粒体 凋亡 |
| 英文关键词:Zhibai Dihuang Decotion ureaplasma urealyticum spermatogenic cell mitochondria apoptosis |
| 基金项目:国家自然科学基金资助项目(No.30973759);湖南省自然科学基金资助项目(No.09JJ3054) |
|
| 摘要点击次数: 1490 |
| 全文下载次数: 1 |
| 中文摘要: |
| 目的观察知柏地黄汤对解脲支原体(UU)感染大鼠生精细胞凋亡及对细胞凋亡线粒体调控效应因子Caspase-3、Caspase-9表达的影响。方法从60只雄性SD大鼠中随机抽取45只,经膀胱注射造成UU感染动物模型,剩余15只作为假手术组。UU感染模型动物再随机分成模型组、美满霉素治疗组和知柏地黄汤治疗组,于接种后10d开始经灌胃给药,模型组和假手术组予生理盐水灌胃,美满霉素治疗组和知柏地黄汤治疗组分别予相应药物灌胃,连续灌胃21天后处死动物,检测各组生精细胞凋亡率、Caspase-3与Caspase-9表达水平和生精细胞超微结构改变。结果与模型组比较,假手术组、美满霉素治疗组和知柏地黄汤治疗组在UU培养阳性率、生精细胞凋亡率、Caspase-3、Caspase-9表达水平差异均有统计学意义(P<0.05),但美满霉素治疗组与知柏地黄汤治疗组比较,差异无统计学意义(P>0.05)。结论 UU感染可致大鼠生精细胞凋亡增加,知柏地黄汤能有效抑制UU的生长、繁殖,具有抗UU作用,知柏地黄汤治疗UU感染、提高精液质量的机制之一是通过调节细胞凋亡效应因子Caspase-3、Caspase-9表达,从而抗细胞凋亡。 |
| 英文摘要: |
| Objective To observe the effects of Zhibai Dihuang Decotion(ZDD) on the ureaplasma urealyticum(UU)-infected rats’ spermatogenic cell apoptosis and expressions of Caspase-3 and Caspase-9.Methods 45 out of 60 male SD rats were randomly selected and made into the UU infected animal model.The rest 15 were taken as the sham-operation group.The UU infected model animals were then randomly divided into the model group,the minocycline group,and the ZDD group.From the 10th day after inoculation,normal saline was given to rats of the model group and the sham-operation group by gastrogavage,while corresponding medicines were given to rats in the minocycline group and the ZDD group.All rats were killed after 21 successive days of gastrogavage.The apoptosis rate of reproductive cells,Caspase-3 and Caspase-9 expression levels and ultramicrostructure changes of spermatogenic cells of each group were detected and compared.Results There was statistical difference in the positive rate of the UU cultivation results,the apoptosis rate of reproductive cells,Caspase-3 and Caspase-9 expression levels in the sham-operation group,the minocycline group,and the ZDD group when compared with the model group(P<0.05).There was statistical difference in the aforesaid indices in the minocycline group and the ZDD group when compared with the sham-operation group(P<0.05).Still there was no statistical difference in the aforesaid indices between the minocycline group and the ZDD group(P>0.05).Conclusions UU infection can lead to the increasing of spermatogenic cell’s apoptosis in rats.ZDD could actively inhibit the growth and production of UU with anti-UU.One of the mechanisms of ZDD in treating UU infection and improving the sperm quality is through regulating the expressions of the apoptosis effect factors Caspase-3 and Caspase-9. |
| 关闭 |
|
|
|
扫描微信二维码，获取更多信息