慢性乙型病毒性肝炎湿热中阻证血浆蛋白质组学分析

作者	单位
魏嵋	泸州医学院中西医结合学院中医诊断学教研室
王小栋	泸州医学院附属中医院肝胆内科
王磊琼	泸州医学院中西医结合学院中医诊断学教研室
刘友平	泸州医学院生物化学教研室
李洪	泸州医学院生物化学教研室
郭芳宏	泸州医学院中西医结合学院中医诊断学教研室

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中文摘要:

目的从蛋白质组学的角度探讨慢性乙型病毒性肝炎(chronic viral hepatitisB,CHB)湿热中阻证的本质内涵。方法采用双向凝胶电泳(2-DE)技术结合质谱分析等生物信息学分析方法对CHB湿热中阻证组(20例)及健康对照组(5名)的血浆全蛋白进行比较蛋白质组学分析。结果 CHB湿热中阻证组2-DE检测获得8个有明显规律性变化的差异表达蛋白点,通过质谱分析鉴定获得7个蛋白点(有1个蛋白点未检出结果):载脂蛋白C2(apoplipoprotein C2,APO-C2)、玻连蛋白(vitronectin,VN)、结合珠蛋白(haptoglobin,HPT)、甲状腺素结合蛋白(transthyretin,TTHY)、载脂蛋白A1(APO-A1)、血清淀粉蛋白P(serum amyloid Pcomponent,SAMP)、载脂蛋白A4(APO-A4),其中与健康对照组比较,APO-A1、APO-A4蛋白点表达量有所升高,其余5个差异蛋白点表达明显下调。结论差异表达蛋白质APO-A1、APO-A4具有潜在的作为CHB湿热中阻证诊断、预后标记物或治疗靶点的意义。

英文摘要:

Objective To study the essence of chronic viral hepatitis B (CHB) of damp-heat retention in the middle-jiao syndrome (DRMS) from plasma proteomic angle. Methods Plasma proteomic analyses of plasmal whole protein of patients in the group with CHB of DRMS (20 cases) and subjects in the health control group (5 cases) were compared using two-dimensional gel electrophoresis (2-DE), mass spectrography, and other bioinformatics analyses methods. Results Eight protein dots with obvious regularity changes of differential expression were obtained by 2-DE. Seven protein dots were obtained by mass spectrography (One protein dot with undetected results): apoplipoprotein C2 (APO-C2), vitronectin (VN), haptoglobin (HPT), transthyretin (TTHY), APO-A1, serum amyloid P-component (SAMP), and APO-A4. Compared with the health control group, the expressions of APO-A1 and APO-A4 were somewhat higher and the expressions of the expressions of the rest five protein dots were obviously down-regulated. Conclusion APO-A1and APO-A4 were of potential significance in the diagnosis of CHB patients of DRMS, prognostic markers, or treatment targets.

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