解毒祛瘀滋肾方对MRL/lpr狼疮小鼠肾组织GRα的调控作用研究

作者	单位
徐莉	浙江中医药大学中医临床基础免疫风湿病实验室
谢志军	浙江中医药大学中医临床基础免疫风湿病实验室
温成平	浙江中医药大学中医临床基础免疫风湿病实验室
季巾君	浙江中医药大学中医临床基础免疫风湿病实验室
范永升	浙江中医药大学中医临床基础免疫风湿病实验室

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中文摘要:

目的探讨解毒祛瘀滋肾方对MRL/lpr小鼠肾组织糖皮质激素受体信号通路的作用机制。方法 30只MRL/lpr小鼠随机分为模型组、西药组和中药组,另选10只昆明鼠作为正常对照组。HE染色分析小鼠肾组织病理改变;RT-PCR和Western blot分析糖皮质激素受体α(GRα)的表达;免疫共沉淀检测GRα与亲环素A结合力的影响。结果两个用药组MRL/lpr小鼠肾损伤程度减轻。与模型组比较,西药组小鼠肾组织GRαmRNA及蛋白相对表达量降低,中药组两个指标增加,差异均有统计学意义(P<0.01)。解毒祛瘀滋肾方可显著提高GRα与亲环素A的结合力。结论上调GRα的表达并增强GRα与亲环素A的相互作用是解毒祛瘀滋肾方能够改善小鼠肾组织病变作用机制之一。

英文摘要:

Objective To explore the action mechanism of Jiedu Quyu Zishen Recipe(JQZR) on the signal transduction of glucocorticoid receptor α(GRα) in the renal tissue of MRL/lpr mice. Methods Thirty MRL/lpr mice were randomly divided into three groups,i.e.,the model group,the Western medicine group,and the Chinese medicine group,10 in each. Besides,another 10 Kunming mice was taken as the normal control group. The pathological changes of the renal tissue were observed using HE staining. The expression of GRα was analyzed using Real-time PCR and Western blot. The effects of JQZR on the binding power of GRα to cyclophilin A were detected using co-immunoprecipitation. Results The renal injury degree of MRL/lpr mice in the Western medicine group and the Chinese medicine group was alleviated. Compared with the model group,the relative quantitation of GRα mRNA and protein expressions in the renal tissue of mice in the Western medicine group decreased,while they increased in the Chinese medicine group,showing statistical difference(P<0.01) . JQZR could significantly elevate the binding potency of GRα to cyclophilin A. Conclusion Up-regulating the expression of GRα and enhancing mutual actions of GRα and cyclophilin A was one of JQZR′s effects on improving the lesion of the renal tissue.

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