补肾化痰复方对多囊卵巢综合征模型大鼠卵巢内胰岛素信号传导分子的调控

作者	单位
吴效科	黑龙江中医药大学附属第一医院妇科实验室
梁晨	黑龙江中医药大学附属第一医院妇科实验室
匡洪影	黑龙江中医药大学附属第一医院妇科实验室
侯丽辉	黑龙江中医药大学附属第一医院妇科实验室
丛晶	黑龙江中医药大学附属第一医院妇科实验室
常惠	黑龙江中医药大学附属第一医院妇科实验室

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中文摘要:

目的观察补肾化痰复方对多囊卵巢综合征(polycystic ovary syndrome,PCOS)模型大鼠糖脂代谢及卵巢内胰岛素信号传导分子表达的影响。方法 Wistar雌性大鼠孕16天时分别在颈背部皮下注射丙酸睾丸酮2.5 mg/kg(其子代雌鼠随机分为给药组与模型组,每组10只)或等剂量中性茶油(其子代雌鼠为对照组,10只),每天1次,连续3天。给药组给予补肾化痰复方灌胃,模型组及对照组给予同体积蒸馏水灌胃,每天1次,连续20天。称大鼠体重、卵巢重量,计算脂体比;采用口服葡萄糖耐量试验(OGTT)测定大鼠0、0.5、1、2 h血糖水平;检测血清高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)、空腹血糖及胰岛素水平,计算稳态模型胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR);采用Western blot法检测卵巢内胰岛素信号传导分子蛋白激酶B(AKT2)、糖原合成酶激酶3β(GSK3β)、葡萄糖转运蛋白4(GLUT4)和细胞外信号调节激酶1(ERK1)的蛋白表达以及AKT2、GSK3β、ERK1磷酸化(P-AKT2、P-GSK3β、P-ERK1)的蛋白表达。结果与对照组比较,模型组脂体比、OGTT试验0.5、2 h血糖水平及HO-MA-IR均明显升高,HDL-C水平明显降低(P<0.05)。与模型组比较,给药组脂体比、OGTT试验2 h血糖水平明显降低,HDL-C水平明显升高(P<0.05)。模型组卵巢组织中AKT2、P-AKT2、GSK3β、P-GSK3β、GLUT4及ERK1的表达明显低于对照组(P<0.05);给药组GSK3β、P-GSK3β及GLUT4的表达较模型组明显增高(P<0.05)。结论 PCOS雌鼠存在胰岛素抵抗及糖脂代谢功能异常,并存在卵巢组织信号传导通路的异常。补肾化痰复方能够改善胰岛素抵抗及糖脂代谢功能,可能与胰岛素信号传导分子蛋白表达的调控有关。

英文摘要:

Objective To study the effects of Bushen Huatan Compound(BHC) on the glucolipid metabolism and the expressions of the insulin signal conducting molecules inside ovaries in polycystic ovary syndrome(PCOS) model rats.Methods Female Wistar rats were subcutaneously injected with 2.5 mg/kg testosterone propionate(Their female offspring were randomly divided into the medication group and the model group,10 in each.) or neutral tea oil of the same dose(Ten female offspring was taken as the control group.) on the 16th day of pregnancy,once daily,for 3 successive days.BHC was given to rats in the medication group by gastrogavage,while equal volume of distilled water was given to rats in the model group and the control group by gastrogavage,both once daily for 20 successive days.The body weight and ovary weight were weighed to calculate the ratio of wet fat weight/body weight.The blood glucose levels were detected at 0,0.5,1,and 2 h using oral glucose tolerance test(OGTT).The serum concentrations of high-density lipoprotein cholesterol(HDL-C),triglyceride(TG),fasting blood glucose(FBG),and insulin were detected to calculate homeostasis model assessment of insulin resistance(HOMA-IR).The expressions of protein kinase B(AKT2),glycogen synthase kinase-3β(GSK3β),glucose transporter-4(GLUT4),extracellular signal regulated kinase-1(ERK1) protein,P-AKT2,P-GSK3β,and P-ERK1 in ovaries were detected using Western blot.Results Compared with the control group,the ratio of wet fat weight/body weight,the blood glucose levels at 0.5 and 2 h in OGTT,and HOMA-IR all obviously increased,and the HDL-C level obviously decreased in the model group(P<0.05).Compared with the model group,the ratio of wet fat weight/body weight and the blood glucose levels at 2 h in OGTT obviously decreased,and the HDL-C level obviously increased in the medication group(P<0.05).The expressions of AKT2,P-AKT2,GSK3β,P-GSK3β,GLUT4,and ERK1 in the ovary tissue were obviously lower in the model group than in the control group(P<0.05).The expressions of GSK3β,P-GSK3β,and GLUT4 were more obviously enhanced in the medication group than in the model group(P<0.05).Conclusions Insulin resistance and glucolipid metabolism dysfunction existed in female PCOS rats.Besides,abnormal insulin signaling pathway existed in the ovary tissue.BHC could remarkably ameliorate the IR degree and glucolipid metabolism functions,and might be correlated with regulating the protein expressions of insulin signal conducting molecules.

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