复方丹参滴丸对代谢综合征模型大鼠胸骨舌骨肌结构和功能的影响

作者	单位
张立强	北京大学第三医院呼吸科
王建东	北京大学医学部生理学系
唐岩	北京市海淀医院高干科
孙兴华	北京大学第三医院呼吸科
李枫	北京市海淀医院高干科
贺蓓	北京大学第三医院呼吸科

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中文摘要:

目的研究复方丹参滴丸对代谢综合征(metabolic syndrome,MS)模型大鼠胸骨舌骨肌结构和功能的影响,探讨其是否对阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea-hypopnea syndrome,OSAHS)具有治疗效应。方法 21只健康雄性SD大鼠随机分为正常对照组(6只)、MS组(8只)和复方丹参滴丸组(7只)。正常对照组常规饲养;MS组和复方丹参滴丸组给予高脂饲料饲养9周后,复方丹参滴丸组加用复方丹参滴丸375 mg/kg灌胃,继续高脂饲养12周。分别测定3组大鼠胸骨舌骨肌丙二醛(malondi-aldehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性;应用肌球蛋白ATP酶组织化学法检测毛细血管密度、毛细血管/肌纤维比率(capillary-to-fiber ratio,C/F)和Ⅰ型肌纤维截面积;测定等长收缩肌条在不同频率电刺激下收缩性能的变化。结果复方丹参滴丸组胸骨舌骨肌MDA含量明显低于MS组,SOD活性、毛细血管密度、C/F、Ⅰ型肌纤维截面积、10～60 Hz电刺激频率下的肌张力、1～5 min肌张力比值均明显高于MS组,差异有统计学意义(P<0.05,P<0.01)。结论复方丹参滴丸可改善由氧化应激反应诱发的MS模型大鼠上气道肌肉内微循环障碍和肌纤维结构变化,提高其收缩性能,可能对OSAHS具有治疗效应。

英文摘要:

Objective To study the effects of Compound Danshen Dripping Pill(CDDP) on the structure and functions of sternohyoid muscle in metabolic syndrome(MS) rats,and to study whether it has therapeutic effects on obstructive sleep apnea-hypopnea syndrome(OSAHS).Methods Twenty-one healthy male SD rats were randomly divided into three groups,i.e.,the normal control group(n=6),the MS group(n=8),and the CDDP group(n=7).Rats in the normal control group were routinely fed.High lipid forage was given to rats in the rest two groups.Nine weeks later,CDDP(at the dose of 375 mg/kg) was additionally given to rats in the CDDP group by gastrogavage,and then rats in the CDDP group and the MS group were fed with the same high lipid forage for 12 successive weeks.The content of malondialdehyde(MDA) and the activities of superoxide dismutase(SOD) in the sternohyoid muscle were detected in the three groups.The capillary density,capillary-to-fiber ratio(C/F),the section area of type Ⅰ muscle fiber were detected using myosin-ATPase histochemical assay.The contractile changes of isometric sternohyoid muscles were determined under electric stimulation by different frequencies.Results The contents of MDA were obviously lower in the CDDP group than in the MS group,while the activities of SOD,the capillary density,C/F,the section area of type Ⅰ muscle fiber,the tension of sternohyoid muscle at 10-60 Hz,and the 1-5 min tension percentages of the sternohyoid muscle were higher in the CDDP group than in the MS group(P<0.05,P<0.01).Conclusion CDDP could improve oxidative stress induced intramusculary microcirculation disturbance and changes of muscular fiber structures of the upper airway muscles,and elevate their contractile functions,thus possibly contributing to favorable effects on OSAHS.

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