生长激素联合续断对去垂体幼龄大鼠生长及骨代谢的影响

作者	单位
赵秋玲	卫生部中日友好医院儿科
张知新	卫生部中日友好医院儿科
张琼	卫生部中日友好医院儿科
苏慧敏	北京市朝阳区妇儿医院儿科
潘琳	卫生部中日友好医院临床研究所
刘应科	卫生部中日友好医院儿科
李鸿	卫生部中日友好医院临床研究所

摘要点击次数: 1944

全文下载次数: 1

中文摘要:

目的探究生长激素(GH)联合中药酒制续断对去垂体幼龄大鼠干预后的促生长作用及骨矿含量(BMC)的影响。方法采用经颅咽去垂体术建立生长激素缺乏大鼠模型,筛选合格动物分为假手术组(正常组,15只)、阴性对照组(模型组,13只)、GH干预组(GH组,13只)、GH联合中药续断组(联合组,12只),GH组和联合组每日相同时间给予GH(0.25 mg/kg)颈部皮下注射,其他组注射等量生理盐水,联合组同时给予续断煎剂(0.7 mL/100 g)灌胃,其他组灌胃等量生理盐水,干预期间测量体重、尾长、身长,干预14天后采血,取股骨、胫骨,检测GH、类胰岛素生长因子I(IGF-1)、碱性磷酸酶(ALP)、骨钙素(OC)等水平,测量胫骨骨骺板宽度,用双能X线测量同侧股骨的骨密度(BMD)及BMC。结果干预2周后,与模型组比较,用药组体重、身长、胫骨长、股骨长、骨骺板宽度值、GH、IGF-1、ALP、OC升高,差异有统计学意义(P<0.01),尾长值增高,但差异无统计学意义(P>0.05),两组间以上指标比较差异无统计学意义(P>0.05);与模型组比较,联合组BMD值升高,差异有统计学意义(P<0.01)。与模型组比较,用药组BMC值升高(P<0.01),联合组最高,差异有统计学意义(P<0.01)。结论 GH联合中药酒制续断对促生长作用不明显,但能提高BMD和BMC,改善骨代谢。

英文摘要:

Objective To study the effect of growth hormone(GH) combined with Radix Dipsaci on the body growth and the bone mineral content(BMC) of hypophysectomized rats.Methods The GH deficiency rats model was established using the hypophysectomized operation through the skull and the throat.Qualified rats were divided into the sham-operation group(n=15),the negative control group(n=13),the GH intervention group(n=13),and the GH combined with Radix Dipsaci group(n=12).GH(0.25 mg/kg) was subcutaneously injected from the cervical part in the GH intervention group and the GH combined with Radix Dipsaci group at the same time,while equal volume of normal saline was injected to the rest groups.0.7 mL/100 kg Radix Dipsaci was given by gastrogavage to the GH combined with Radix Dipsaci group at the same time,while equal volume of normal saline was given by gastrogave to the rest groups.The body weight,the tail length,and the body length were measured during the intervention period.Blood was withdrawn after 14-day intervention.The femoral bone and the tibial bone were taken out.The levels of GH,insulin-like growth factor Ⅰ(IGF-1),alkaline phosphatase(ALP),and osteocalcin(OC) were measured.The width of the tibial epiphyseal plate was measured.The bilateral femur bone mineral density(BMD) and BMC were measured using the dual energy X-ray absorptiometry.Results The body weight,the body length,the length of the femoral bone,the length of the tibial bone,the width of the epiphyseal plate,the levels of the GH,IGF-1,ALP,and OC increased in the GH intervention group and the GH combined with Radix Dipsaci group after 2-week intervention,showing statistical difference when compared with the model group(P<0.01).But there was no statistical difference in the tail length though it also increased(P>0.05).There was insignificant difference in the aforesaid indices between the two groups(P>0.05).Compared with the model group,the BMD of the GH combined with Radix Dipsaci group increased with statistical difference(P<0.01).Compared with the model group,the BMC of the GH intervention group and the GH combined with Radix Dipsaci group increased with statistical difference(P<0.01).It was highest in the GH combined with Radix Dipsaci group(P<0.01).Conclusions GH combined with Radix Dipsaci showed unobvious effect on promoting the growth.But it could elevate BMD and BMC,and improve the bone metabolism.

关闭