生血增白汤对骨髓移植后小鼠骨髓细胞周期和细胞凋亡的影响

目的探讨生血增白汤对骨髓移植后小鼠骨髓中细胞周期和细胞凋亡的影响及其促进骨髓造血重建的机制。方法将实验动物随机分为5组:正常组、模型组、粒细胞-集落刺激因子组(G-CSF组)、生血组、生血+G-CSF组(联合组);建立小鼠骨髓移植模型,生血组及联合组胃饲生血增白汤浓缩煎剂。骨髓移植后第9、14、24天,采用流式细胞仪检测骨髓单核细胞凋亡率(APO)并分析细胞周期变化,进行外周血细胞和骨髓单核细胞计数,并做骨髓组织学观察。结果骨髓移植后第14天,生血组外周血细胞中血红蛋白高于模型组及G-CSF组(P<0.05),联合组高于模型组(P<0.05);骨髓移植后第24天,生血组外周血细胞中白细胞高于模型组(P<0.05)。骨髓移植后第24天时,G-CSF组、生血组和联合组骨髓单核细胞计数均高于模型组(P<0.01)。骨髓移植后第24天,生血组第24天APO明显低于同期模型组及G-CSF组(P<0.01)。结论生血增白汤能促进骨髓造血细胞的增殖分化,抑制骨髓细胞凋亡,可能是生血增白汤重建造血的机制之一。

英文摘要:

Objective To explore the effect of Shengxue Zengbai Decoction on the cell cycle and apoptosis in hematopoietic rebuilding in mice after bone marrow transplantation(BMT).Methods The experimental animals were randomly divided into 5 groups:the normal group(with no treatment),the model group,the granulocyte-colony stimulating factor(G-CSF) group,the Shengxue group,and the Shengxue+ G-CSF group.BMT mouse model was established.Shengxue Zengbai Decoction concentrated decoction was given by gastrogavage to mice in the Shengxue group and the Shengxue+ G-CSF group.On Day 9,14,and 24 after BMT,the apoptotic rates of myelomonocytes were detected using flow cytometry.Changes of the cell cycle were analyzed.Peripheral blood cells and myelomonocyte were counted.Histological observation was performed.Results On Day 14 after BMT the level of hemoglobin in the peripheral blood cells was higher in the Shengxue group than in the model group and the G-CSF group(P<0.05).The level of hemoglobin in the Shengxue+G-CSF group was higher than that in the model group(P<0.05).On Day 24 after BMT the level of white blood cells in the peripheral blood cells in the Shengxue group was higher than that in the model group(P<0.05).On Day 24 after BMT the myelomonocyte count in the G-CSF group,the Shengxue group,and the Shengxue+G-CSF group was higher than in the model group(P<0.01).The cell apoptotic rate on Day 24 after BMT was lower in the Shengxue group than in the G-CSF group and the model group of the same period.Conclusion Shengxue Zengbai Decoction could promote the proliferation and differentiation of bone marrow hematopoietic cells,inhibit the apoptosis of bone marrow cells,which might be one of the mechanisms for hematopoietic rebuilding of Shengxue Zengbai Decoction.

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