人参三七组方对大鼠缺血心肌Ras信号通路的影响

目的探讨人参三七组方对急性心肌梗死(acute myocardial infarction,AMI)大鼠缺血心肌Ras、细胞外信号调节激酶1/2(extracellular signal-regulated kinase1/2,ERK1/2)和C-Raf-1蛋白及mRNA表达的影响。方法采用Wistar大鼠冠状动脉左前降支结扎的心肌缺血模型,并设空白组、假手术组、模型组、倍他乐克组和人参三七组方大、小剂量组。通过Western blot法检测缺血心肌的Ras、ERK1/2、磷酸化ERK1/2(phosphor-ERK1/2,p-ERK1/2)及C-Raf-1的蛋白含量,Real-time PCR法检测缺血心肌Ras、ERK1、ERK2及C-Raf-1mRNA表达情况。结果模型组Ras、C-Raf-1、p-ERK1/2蛋白及其mRNA表达较空白组、假手术组增加(P<0.05);人参三七组方大、小剂量组及倍他乐克组Ras、C-Raf-1、p-ERK1/2蛋白及其mRNA表达均显著高于模型组(P<0.05);人参三七组方大剂量组较小剂量组Ras、C-Raf-1、p-ERK1/2蛋白及其mRNA表达增高更明显(P<0.05)。各组ERK1/2蛋白表达差异无统计学意义(P>0.05)。结论人参三七组方能够促进缺血心肌Ras信号通路上的关键靶点Ras、C-Raf-1及ERK1/2表达,提示人参三七组方可能通过Ras信号通路来促进血管新生,具有一定剂量依赖性。

英文摘要:

Objective To study the effects of Ginseng and Sanchi Compositions(GSC) on the protein and mRNA expressions of Ras,extracellular signal-regulated Kinase1/2(ERK1/2),and C-Raf-1 of ischemic myocardium of rats with acute myocardial infarction(AMI).Methods By adopting myocardial ischemia Wistar rat model with the ligation of the left anterior descending coronary artery,the normal control group,the sham-operation group,the model group,the Betaloc group,the high and low dose GSC group were set up.The protein expressions of Ras,C-Raf-1,ERK1/2,and phosphor-ERK1/2,p-ERK1/2(p-ERK1/2) were detected by Western blot.The mRNA expressions of Ras,C-Raf-1,ERK1,and ERK2 were detected using Real-time PCR.Results The protein expressions of Ras,c-Raf-1,and p-ERK1/2 and their mRNA expressions in the model group increased more than those in the normal control group and the sham-operation group(P<0.05).The protein expressions of Ras,c-Raf-1,and p-ERK1/2 and their mRNA expressions in the high and low dose GSC groups and the Betaloc group were significantly higher than those in the model group(P<0.05).The protein expressions of Ras,c-Raf-1,and p-ERK1/2 and their mRNA expressions increased more obviously in the high dose GSC group than in the low dose GSC group(P<0.05).The ERK1/2 protein expression was not significantly different among all groups(P>0.05).Conclusions GSC could up-regulate the protein expressions and mRNA expressions of Ras,C-Raf-1,and p-ERK1/2.It suggested that GSC might promote the angiogenesis through Ras signal transduction pathway dose-dependently.

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