| 高冬,焦雨欢,武一曼,林凡,陈岩,逯波,宋军,陈可冀.血府逐瘀汤诱导内皮祖细胞参与缺血区血管新生的实验研究[J].中国中西医结合杂志,2012,32(2):224-228 |
| 血府逐瘀汤诱导内皮祖细胞参与缺血区血管新生的实验研究 |
| Experimental Study of Xuefu Zhuyu Decoction Induced Participation of Endothelial Progenitor Cells in the Angiogenesis of the Ischemic Region |
| 免费下载全文 查看/发表评论 下载PDF阅读器 |
| |
| DOI： |
| 中文关键词: 血府逐瘀汤 内皮祖细胞 血管新生 |
| 英文关键词:Xuefu Zhuyu Decoction endothelial progenitor cell angiogenesis |
| 基金项目:国家自然科学基金资助项目(No.30772877);北京市自然科学基金资助课题(No.7122120);中国中医科学院基本科研业务费自主选题项目(No.ZZ2006039);福建中西医结合研究院科研基金项目(No.ZX05001) |
|
| 摘要点击次数: 2134 |
| 全文下载次数: 15 |
| 中文摘要: |
| 目的探讨血府逐瘀汤影响缺血区血管生长和修复损伤的作用。方法采用血管栓塞法制作下肢缺血大鼠模型,将DAPI荧光标记的内皮祖细胞由尾静脉注射植入模型鼠体内,继而给予不同剂量血府逐瘀汤灌胃,于给药第3天和第7天,分别采血并取材缺血坏死部位肉芽组织和肌肉组织,行冰冻切片分析荧光表达,常规病理切片观察各组样本坏死病变情况并计数局部血管数量。硝酸还原酶法检测血清NO水平。缺血下肢大体观察延续至30天。结果给药3天和7天,高剂量药物组缺血区荧光强度和肉芽组织血管数量均明显高于常规剂量药物组和生理盐水组,给药7天后常规剂量药物组的上述指标也显著高于生理盐水组;另外高剂量及常规剂量药物组两个时相点血清NO含量均明显高于生理盐水组。给药30天,高剂量药物组造模下肢肌肉萎缩现象最不显著。结论血府逐瘀汤通过提高NO水平诱导内皮祖细胞迁移至缺血区,促进血管新生,进而改善缺血坏死。 |
| 英文摘要: |
| Objective To observe the effects of Xuefu Zhuyu Decoction(XFZYD) on the angiogenesis and injury repair in the ischemic region.Methods The ischemic hind limb rat model was established using Bletilla embolization.Endothelial progenitor cells(EPCs) were labeled with DAPI and injected into the model rats from the vena caudalis.Then rats were treated with different doses of XFZYD by gastrogavage.Blood was withdrawn.The granulation tissue and the muscle tissues from ischemic and necrotic portion were taken on the 3rd and 7th day of the medication.The samples were frozen and sliced to analyze the fluorescent expressions.The necrosis of each sample was observed by routine pathological section.The vessels number was counted.The serum NO levels were detected using nitrate reductase method.The macro-morphological observation of ischemic lower limbs were lasted for 30 days.Results After 3 and 7 days of medication,the fluorescence intensity of ischemic area and the number of granulation vessels were significantly more in the high dose XFZYD group than in the routine treatment group and the normal saline treatment groups.The aforesaid indices were significantly higher in the routine treatment group than in the normal saline treatment group after 7 days of medication.The serum NO concentrations were significantly lower in the normal saline group at other time points.On the 30th day of medication,the muscular atrophy of the ischemic hind limbs was the least significant in the high dose XFZYD group.Conclusion XFZYD could improve the ischemic necrosis by improving the NO level,inducing the EPCs′ migration to the ischemic region,and promoting the angiogenesis. |
| 关闭 |
|
|
|
扫描微信二维码，获取更多信息