复方肾华片对糖尿病肾病大鼠肾脏保护作用研究

作者	单位
耿文佳	解放军总医院肾病科肾脏病国家重点学科暨重点实验室
魏日胞	解放军总医院肾病科肾脏病国家重点学科暨重点实验室
毛炜	广东省中医院肾内科
刘旭生	广东省中医院肾内科
师锁柱	解放军总医院肾病科肾脏病国家重点学科暨重点实验室

摘要点击次数: 1866

全文下载次数: 13

中文摘要:

目的观察复方肾华片对糖尿病肾病大鼠的肾脏保护作用。方法采用在大鼠单侧左肾切除基础上给予腹腔注射链脲佐菌素(65mg/kg)造模,造模后正常饮食9个月糖尿病肾病大鼠模型制备成功。分为正常对照组、模型对照组、复方肾华片组和厄贝沙坦组,给予6周灌胃干预治疗。观察干预前后大鼠一般状态及24h尿蛋白定量、尿微量白蛋白、血肌酐(SCr)、血尿素氮(BUN)、血糖(GLU)、甘油三酯(TG)、总胆固醇(TC)、总蛋白(TB)、白蛋白(ALB)水平;进行肾组织PAS染色及透射电镜观察。结果药物干预6周后,与模型对照组比较,厄贝沙坦组及复方肾华片组大鼠一般情况均改善;两治疗组大鼠24h尿蛋白定量、尿微量白蛋白、SCr、BUN、GLU、TG和TC均较模型组及本组治疗前明显降低(P<0.05,P<0.01),两治疗组比较差异无统计学意义(P>0.05);两治疗组肾脏病理及肾脏超微结构均较模型对照组有不同程度改善。结论复方肾华片具有减轻糖尿病肾损害,延缓肾脏功能损害进展的作用,疗效与厄贝沙坦相当。

英文摘要:

Objective To observe the renal protection effects of Compound Shenhua Tablet(CST) on diabetic nephropathy(DN) rats.Methods DN rats were given a normal diet for 9 months after they were induced by intraperitoneal injection of STZ at the dose of 65 mg/kg after uninephrectomized.They were randomly divided into 4 groups,i.e.,the normal control group,the model control group,the CST group,and the Irbesartan group.The intervention was given by gastrogavage for 6 weeks.The general state,24 h urine protein,urine micro-albumin(mAlb) ,serum creatinine(SCr) ,blood urea nitrogen(BUN) ,glucose(GLU) ,triglyceride(TG) ,total cholesterol(TC) ,total protein(TP) ,and albumin(ALB) levels were observed before and after intervention.Renal pathological changes were observed by PAS staining and transmission electron microscope.Results After 6 weeks of drug intervention,when compared with the model control group,the general state was improved in the CST group and the Irbesartan group.The levels of 24 h urine protein,urine mAlb,SCr,BUN,GLU,TG,and TC were obviously lower in the CST group and the Irbesartan group than in the model group as well as in the same group before treatment(P<0.05,P<0.01) .There was no statistical difference between the two treatment groups(P>0.05) .The renal pathological changes and the renal ultrastructure were improved to some degree in the two groups when compared with those in the model control group.Conclusions CST could attenuate the renal damage of diabetes and delay renal deterioration process.Its effectiveness was equivalent to that of Irbesartan.

关闭