活血解毒方对糖尿病大鼠视网膜病变的影响

作者	单位
姚青	北京中医药大学基础医学院
韩静	宁夏医科大学基础医学院
余俊达	宁夏医科大学基础医学院
黄黎明	宁夏医科大学基础医学院
潘琳	卫生部中日友好医院临床研究所
王伟	宁夏医科大学基础医学院

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中文摘要:

目的探讨活血解毒方对糖尿病大鼠视网膜中央动脉(central retinal artery,CRA)和中央静脉血流动力学及血管形态的影响。方法选取SD大鼠60只,禁食12h,一次性腹腔注射链脲佐菌素(streptozotocin,STZ,65mg/kg)诱导糖尿病模型。造模后将大鼠随机分为模型组,活血解毒方高(15.4g/kg)、中(7.70g/kg)、低(3.85g/kg)剂量组及羟苯磺酸钙胶囊组(西药组,0.167g/kg),每组10只。另选取10只大鼠作为正常对照组(正常组),给予等体积蒸馏水灌胃。每组每天干预1次,共20周。检测各组大鼠CRA收缩期峰值血流速度(peak systolic velocity,PSV)、舒张期末期流速(end diastolic velocity,EDV)、平均血流速度(mean velocity,MV)、搏动指数(plusitive index,PI)、阻力指数(resistive index,RI)及视网膜中央静脉血流速度(central retinal vena velocity,CRV),应用视网膜消化铺片方法,观察并比较各组大鼠视网膜血管形态。结果与正常组比较,模型组PSV、EDV、MV及CRV降低,PI、RI、毛细血管面积密度升高,差异均有统计学意义(P<0.01),视网膜毛细血管排列紊乱,毛细血管管径粗细不均匀,可见内皮细胞增生,周细胞减少。与模型组比较,活血解毒方高、中剂量组PSV、EDV、MV及CRV均升高,PI、RI均降低,差异均有统计学意义(P<0.01),各用药组间比较,差异无统计学意义(P>0.05)。活血解毒方各剂量组毛细血管的分布较均匀,血管形态较规则,未见明显扭曲和扩张,内皮细胞增生状况不明显,与模型组比较,毛细血管面积密度显著下降(P<0.01)。活血解毒方各剂量组间比较,差异无统计学意义,与模型组比较,西药组毛细血管面积密度显著下降(P<0.01)。结论活血解毒方能明显改善糖尿病大鼠视网膜血流动力学参数,增加视网膜毛细血管血流量和灌流量,抑制毛细血管内皮细胞的增生。

英文摘要:

Objective To study the effects of Huoxue Jiedu Recipe(HJR) on the hemodynamics of central retinal artery(CRA) and central retinal vena,as well as the morphology of blood vessels of diabetic rats.Methods Sixty SD rats were selected and fasted for 12 h.Streptozotocin(STZ,65 mg/kg) was intraperitoneally injected to induce diabetic rat models.The modeled rats were randomly divided into the model group,the high dose HJR group(15.4 g/kg) ,the middle dose HJR group(7.70 g/kg) ,the low dose HJR group(3.85 g/kg) ,and the Doxium Capsule group(the Western medicine group,0.167 g/kg) ,10 in each group.Another 10 rats were recruited as the normal control group.Equal volume of distilled water was given to rats in the normal control group.The intervention was carried out once daily in each group,totally for 20 weeks.The peak systolic velocity(PSV) ,the end diastolic velocity(EDV) ,the mean velocity(MV) ,the pulsitive index(PI) ,the resistive index(RI) ,and the central retinal vena velocity(CRV) were detected in each group.The retinal vascular morphologies were observed and compared using trypsin digestion.Results Compared with the normal control group,the PSV,EDV,MV,and CRV decreased,PI,RI,and capillary density increased in the model group with statistical difference(P<0.01) .The retinal capillaries rowed disorderly.The calibers of capillaries were not even.The hyperplasia of endothelial cells and less pericytes could be seen.Compared with the model group,PSV,EDV,MV,and CRV all increased,PI and RI decreased in the high and middle dose HJR groups with statistical difference(P<0.01) .There was no statistical difference among all the medication groups(P>0.05) .The distributions of capillaries in the 3 HJR groups were even.The vascular morphous was comparatively regular,without obvious twisting and dilation.The hyperplasia of endothelial cells was not obvious.Compared with the model group,the capillary density significantly decreased(P<0.01) .There was no statistical difference among the 3 HJR groups.Compared with the model group,the capillary density significantly decreased in the Western medicine group(P<0.01) .Conclusion HJR could obviously improve the retinal hemodynamics parameters of diabetic rats,increase the retinal capillary blood flow and reperfusion,and restrain the hyperplasia of endothelial cells in the capillary.

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