狼疮平颗粒对BXSB狼疮性肾炎小鼠B淋巴细胞活化因子表达的影响

Effects of Langchuangping Granule on the Expression of B Lymphocyte Activating Factor in the Peripheral Blood B Cells of BXSB Lupus Nephritis Mice

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中文关键词: 狼疮平颗粒 B淋巴细胞活化因子 BAFF mRNA 狼疮性肾炎

英文关键词:Langchuangping Granule B lymphocyte activating factor BAFF mRNA lupus nephritis

基金项目:河北省科学技术厅基金资助项目(No.10276105D-68);邯郸市科学技术局基金资助项目(No.1023108102)

作者	单位
李桂英	河北工程大学附属医院肾内科
刘淑霞	河北医科大学病理教研室
路新卿	河北工程大学附属医院肾内科
张增海	河北工程大学附属医院肾内科
张军丽	河北工程大学附属医院肾内科
李英	河北医科大学第三医院
王春来	河北省唐山市丰润区中医院

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中文摘要:

目的研究B淋巴细胞活化因子(Blymphocyte activating factor,BAFF)在BXSB狼疮性肾炎小鼠血清和外周血B淋巴细胞(PBBC)中的表达,并观察中药狼疮平颗粒的治疗作用。方法将11周龄雄性BXSB狼疮性肾炎小鼠18只随机分为狼疮对照组、激素治疗组、狼疮平治疗组,每组6只;另取11周龄雄性C57BL/6小鼠6只作为正常对照组。分别给予灌胃生理盐水(10mL/d)、甲泼尼龙[5mg/(kg·d)]、狼疮平颗粒[4g/(kg·d)]、生理盐水(10mL/d),治疗4周,测定各组尿蛋白、ds-DNA和体重,分别应用ELISA、RT-PCR方法检测血清中可溶性BAFF(sBAFF)和外周血B细胞中BAFF-mRNA的表达及变化,并观察肾脏病理活动性计分、24h尿蛋白定量、ds-DNA与sBAFF的关系。结果狼疮性肾炎小鼠血清sBAFF、PBBC中BAFF mRNA水平、24h尿蛋白定量、血清ds-DNA含量均较正常小鼠明显升高。激素和狼疮平治疗组治疗后sBAFF、BAFF mRNA较治疗前明显下降,差异有统计学意义(P<0.05),两组治疗后sBAFF、BAFF mRNA水平无明显差异(P>0.05)。狼疮性肾炎小鼠血清sBAFF水平与肾病理活动性计分、24h尿蛋白定量、ds-DNA成正相关(r=0.8098、0.8220、0.8535,P<0.01)。结论 BAFF在狼疮性肾炎的发生发展中起着重要的作用,可以监测病情变化并预测复发,是狼疮性肾炎治疗的理想靶点之一。中药狼疮平可以通过降低BAFF水平而改善狼疮性肾炎病情,值得进一步临床推广应用。

英文摘要:

Objective To explore the expressions of B lymphocyte activating factor(BAFF) in the serum and peripheral blood B cells(PBBCs) of BXSB lupus nephritis mice,and to investigate the efficacy of Langchuangping Granule(LG) .Methods Eighteen 11-week-old male BXSB lupus mice were randomly divided into three groups,i.e.,the lupus control group,the hormone treatment group,and the LG treatment group,6 in each group.Besides,another 6 C57BL/6 male mice were recruited as the normal control group.The mice were given with normal sodium(10 mL/d) ,methylprednisolone(at the daily dose of 5 mg/kg) ,LG(at the daily dose of 4 g/kg) ,and the normal saline(10 mL/d) respectively by gastrogavage for 4 weeks.The urine protein,ds-DNA,and body weight were determined.The serum soluble BAFF(sBAFF) ,the expressions and changes of BAFF-mRNA in the PBBCs were detected using ELISA and RT-PCR respectively.The activity index(AI) and 24 h urine albumin excretion quantitation of renal pathological activities were observed.The correlation between ds-DNA and sBAFF were analyzed.Results The level of sBAFF in serum,the BAFF mRNA level in PBBCs,24 h urinary albumin excretion,and serum ds-DNA content increased more obviously in lupus mice than in the normal mice.After being treated by methylprednisolone or LG,the sBAFF and BAFF mRNA expressions decreased more obviously than before treatment,showing statistical difference(P<0.05) .But there was no statistical difference in the sBAFF level or the BAFF mRNA expression(P>0.05) .There was positive correlation between sBAFF and AI(r=0.8098,P<0.01) ,24 h urinary albumin excretion(r=0.8220,P<0.01) ,and ds-DNA(r=0.8535,P<0.01) .ConclusionsBAFF plays an important role in the occurrence and development of lupus nephritis.It can be used in monitoring the disease progress and predicting its recurrence.It is one of ideal targets for treating lupus nephritis.LG could attenuate the renal injury via suppressing BAFF level.It is worth further clinical application.

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