知柏地黄汤对解脲支原体感染大鼠睾丸组织病理变化与超微结构的影响

Effects of Zhibai Dihuang Decoction on the Pathological Changes and the Ultrastructure of the Testicular Tissue in the Ureaplasma Urealyticum-infected Rats

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DOI：

中文关键词: 知柏地黄汤解脲支原体病理变化超微结构

英文关键词:Zhibai Dihuang Decoction ureaplasma urealyticum pathological change ultrastructure

基金项目:国家自然科学基金资助(No.30973759);湖南省科学基金资助(No.09JJ3054)

作者	单位
卢芳国	湖南中医药大学基础医学院病原与免疫教研室
何清湖	湖南中医药大学中西医结合学院临床医学教研室
刘朝圣	湖南中医药大学中西医结合学院临床医学教研室
赵建业	湖南中医药大学中西医结合学院临床医学教研室
李玲	湖南中医药大学基础医学院病原与免疫教研室

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中文摘要:

目的探讨知柏地黄汤对解脲支原体(ureaplasma urealyticum,UU)感染大鼠睾丸组织病理变化和超微结构的影响。方法经膀胱接种建立大鼠睾丸组织感染解脲支原体动物模型,将45只UU感染模型动物随机分为:知柏地黄汤治疗组[中药组,2g/(100g·d)]、美满霉素治疗组[西药组,10mg/(100g·d)]及模型组,每组15只。另选取15只大鼠作为假手术组。于动物接种10天后开始给药,假手术组及模型组灌胃等容积生理盐水,连续灌胃22天。于停药后第2天处死动物,检测睾丸重量指数,分别以光学显微镜和透射电子显微镜观察睾丸组织的病理变化与超微结构。结果各组大鼠左、右侧睾丸重量指数、睾丸重量指数比较,差异均无统计学意义(P>0.05)。UU感染可以引起曲细精管萎缩,生精细胞脱落,管腔中精子细胞减少等病理变化与生精细胞核膜皱缩,细胞核破裂,线粒体明显水肿等超微结构改变,用药组病理变化和超微结构得到改善。模型与假手术不重叠,差异有统计学意义(P<0.05);模型与中药和西药不重叠,差异有统计学意义(P<0.05);中药和西药两组95%区间重叠,差异无统计学意义(P>0.05)。结论 UU感染可引起睾丸组织器官水平、细胞水平的病理变化及超微结构的改变,知柏地黄汤能通过改善其病理变化和生精细胞超微结构而发挥治疗作用。

英文摘要:

Objective To study the effects of Zhibai Dihuang Decocion(ZDD) on the pathological changes and the ultrastructure of the testicular tissue in the ureaplasma urealyticum(UU) -infected rats.Methods The UU infected animal models were established by the bladder inoculation.The 45 UU infected SD rats were randomly divided into four groups,i.e.,the ZDD treatment group(at the daily dose of 2 g/100 g) ,the Minocycline group(at the daily dose of 10 mg/100 g) ,the model group,15 in each group.Besides,another 15 rats were recruited as the sham-operation group.The medication was started 10 days after vaccination.Equal volume of normal saline was given to rats in the model group and the sham-operation group by gastrogavage for 22 successive days.Rats were sacrificed on the 2nd day of medication discontinuation.The testicle mass index was detected.The ultra-structure and the pathological changes of the testicular tissue were observed by optical microscope and transmission electron microscope.Results There was no significant difference in the rat testicular mass index(P>0.05) .UU infection can lead to the pathological changes such as atrophy of seminiferous tubules,germ cell loss,and reduction of sperm cells in lumen,and to the ultrastructural changes such as spermatogenic cell nuclear membrane shrinkage,nuclear breakdown,and obvious edema of mitochondria.The pathological changes and the ultrastructures were improved in the medication groups.Rm and Rs the were not overlapping,and the difference was statistically significant(P<0.05) .Rm,Rzh and Rx were not overlapping,and the difference was statistically significant(P<0.05) .Rzh and Rx were overlapping in 95% CI with no statistical difference(P>0.05) .Conclusions UU infection can cause the pathological changes and the ultrastructural changes of the testicular tissue at the organic level and the cellular level.ZDD played therapeutic effects through ameliorating its pathological changes and the ultrastructural changes of spermatogenic cells.

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