比较评价当归川芎配伍对急性血瘀大鼠血液流变学及凝血功能的影响

Comparative Assessing the Effects of Angelica Root and Chuanxiong on the Hemorheology and the Blood Coagulation Function in Acute Blood Stasis Rats

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中文关键词: 当归川芎药对血瘀血液流变凝血功能

英文关键词:angelica root chuanxiong herb pair blood stasis hemorheology blood coagulation function

基金项目:国家科技支撑计划(No.2008BAI51B01);2011年度高等学校博士学科点专项科研基金(No.20113237110010);国家自然科学基金(No.30873235);江苏省自然科学基金(No.BK2008455);江苏省高校自然科学重大基础研究项目(No.06KJA36022,07KJA36024,10KJA36023);国家教育部“新世纪优秀人才支持计划”(No.NCET-09-0163);江苏高校优势学科建设工程资助项目(No.ysxk-2010)

作者	单位
李伟霞	南京中医药大学江苏省方剂高技术研究重点实验室
唐于平	南京中医药大学江苏省方剂高技术研究重点实验室
郭建明	南京中医药大学江苏省方剂高技术研究重点实验室
黄美艳	南京中医药大学江苏省方剂高技术研究重点实验室
钱大玮	南京中医药大学江苏省方剂高技术研究重点实验室
段金廒	南京中医药大学江苏省方剂高技术研究重点实验室

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中文摘要:

目的比较评价当归川芎药对配伍对急性血瘀大鼠血液流变学和凝血功能的影响。方法采用冰水浴和皮下注射盐酸肾上腺素共同复制急性血瘀大鼠模型,通过测定全血黏度、血浆黏度、血沉和血压积,观察当归川芎配伍对血瘀大鼠血液流变学的影响;通过测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和血浆纤维蛋白原(FIB)含量,观察当归川芎配伍对血瘀大鼠凝血功能的影响。最后采用权重系数法将所有指标整合为总活血效应值,来评价当归川芎配伍的总活血化瘀效应。结果与正常组比较,模型组各血液流变学指标明显升高;PT、APTT时间明显缩短,FIB含量明显升高。与模型组比较,当归、川芎单味药及当归川芎药对1:1和1.5:1的水提物、醇提物和先水提后醇提物给药均能降低血液流变学各指标,对PT和APTT时间有显著延长作用,对FIB含量有显著降低作用。提取方法相同时,当归川芎药对1:1醇提物给药组的总活血效应为最好,且川芎单味药的总活血效应优于当归。配比相同的情况下,醇提给药组的总活血效应优于水提和先水提后醇提给药组。结论当归川芎配伍能明显改善血瘀大鼠的血液流变学及凝血功能异常;为揭示当归川芎药对相使配伍的科学内涵提供了依据。

英文摘要:

Objective To comparatively assess the effects of angelica root and chuanxiong on the hemorheology and the blood coagulation function in acute blood stasis rats.Methods Ice water bath and subcutaneous injection of adrenaline were both used to establish the acute blood stasis rat model.The whole blood viscosity,plasma viscosity,erythrocyte sedimentation rate(ESR),and haematocrit were tested to observe the effects of angelica root and chuanxiong on the hemorheology of blood stasis rats.The prothrombin time(PT),activated partial thromboplastin time(APTT),and plasma fibrinogen(FIB) were tested to observe the effects of angelica root and chuanxiong on the blood coagulation function of blood stasis rats.Finally all those indices were integrated to the total activating blood circulation effect value to assess the total effects of angelica root and chuanxiong on activating blood circulation and dissipating blood stasis.Results Compared with the normal control group,the hemorheological indices obviously increased,PT and APTT were obviously shortened,and the FIB content obviously increased in the model group.Compared with the model group,the water extracts,alcohol extracts,water-alcohol extracts of angelica root,chuanxiong,angelica root and chuanxiong 1:1,and angelica root and chuanxiong 1.5:1 could decrease the hemorheological indices,prolong the PT and APTT,and significantly reduce the content of FIB.Under the condition of the same preparation method,the alcohol extract of angelica root and chuanxiong 1:1 showed the best total effect.As for the single herbs,chuanxiong had better effect than angelica root on the total effects of activating blood circulation and dissipating blood stasis.Under the condition of the same ratio,the alcohol extracts of angelica root and chuanxiong showed better total effects of activating blood circulation and dissipating blood stasis than water extracts and water-alcohol extracts.ConclusionThe angelica root and chuanxiong could obviously improve the abnormality of hemorheology and blood coagulation function in acute blood stasis rats,thus providing evidence for revealing the scientific innovation of the compatibility of angelica root and chuanxiong.

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