补肺健脾方对慢性阻塞性肺疾病大鼠膈神经放电和膈肌功能的影响

Effects of Bufei Jianpi Recipe on the Diaphragmatic Neural Discharge and the Diaphragmatic Muscle Function in Rats with Chronic Obstructive Pulmonary Disease

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中文关键词: 补肺健脾方慢性阻塞性肺疾病膈神经放电膈肌功能

英文关键词:Bufei Jianpi Recipe chronic obstructive pulmonary disease diaphragmatic neural discharge diaphragmatic muscle function

基金项目:国家自然科学基金资助项目(No.30973743);2010年度河南省科技创新人才项目(No.104200510004)

作者	单位
李素云	河南中医学院第一附属医院呼吸病研究
李亚	河南中医学院第一附属医院呼吸病研究
李建生	河南中医学院老年医学研究所
邓丽	河南中医学院老年医学研究所
田燕歌	河南中医学院老年医学研究所
姜素丽	河南中医学院老年医学研究所
王英	河南中医学院老年医学研究所

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中文摘要:

目的观察补肺健脾方对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期大鼠膈神经放电和膈肌功能的影响。方法将大鼠随机分为正常对照组、模型组、补肺健脾高、中、低剂量[9.68、4.84、2.42g/(kg·d)]组和氨茶碱组[2.3mg/(kg·d)],采用香烟熏吸联合细菌感染法制作COPD稳定期模型。于第9周起分别给予高、中、低剂量补肺健脾方和氨茶碱至第20周取材,观察肺功能、膈神经放电时间(di-aphragmatic neural discharge time,Td)、间期(diaphragmatic neural discharge interval,Tdi)、幅度(diaphragmaticneural discharge range,Rd)、面积(diaphragmatic neural discharge area,Ad)、吸气时间(inspiratory time,Tin)和呼气时间(expiratory time,Tex)、呼吸频率(respiratory rate,RR)、幅度(respiratory excursion,RE)和面积(respiratoryarea,RA)、膈肌张力及耐力等。结果与正常对照组比较,模型组潮气量(tidal volume,TV)、呼气峰流速(peakexpiratory flow,PEF)和50%潮气量呼气流速(50%tidal volume expiratory flow,EF50)显著降低(P<0.01),Td、Tdi、Tin和Tex显著延长(P<0.05,P<0.01),Rd和Ad、RR、RE和RA、离体膈肌张力与耐力均明显降低(P<0.05,P<0.01),膈肌Ⅰ、ⅡA型纤维比例显著增加(P<0.01),ⅡB型纤维比例显著减少(P<0.01),三磷酸腺苷(a-denosine triphosphate,ATP)酶活性降低而琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性升高(P<0.01)。补肺健脾组上述指标均不同程度改善,尤以高、中剂量组改善较为明显(P<0.05,P<0.01),部分指标疗效优于氨茶碱。结论补肺健脾方可以显著改善COPD大鼠膈神经放电和膈肌功能。

英文摘要:

Objective To observe the effects of Bufei Jianpi Recipe(BJR) on the diaphragmatic neural discharge and the diaphragmatic muscle function in rats with chronic obstructive pulmonary disease(COPD).Methods Rats were randomly divided into the normal control group,the model group,the high dose BJR group(9.68 g/kg·d-1),the medium dose BJR group(4.84 g/kg·d-1),the low dose BJR group(2.42 g/kg·d-1),and the aminophyline group(2.3 mg/kg·d-1).The stable phase COPD rat model was prepared using repeated smoke inhalations and bacterial infections.The high,medium,and low dose BJR and aminophyline was respectively administered to rats from the ninth week to the twentieth week.The sampling was taken.The lung function,diaphragmatic neural discharge time(Td),and diaphragmatic neural discharge interval(Tdi),diaphragmatic neural discharge range(Rd),diaphragmatic neural discharge area(Ad),expiratory time(Tex),inspiratory time(Tin),respiratory rate(RR),respiratory excursion(RE),respiratory area(RA),and diaphragmatic muscular tension and endurance were detected.Results Compared with the normal control group,the tidal volume(TV),peak expiratory flow(PEF),and 50% tidal volume expiratory flow(EF50) significantly decreased in the model group(P<0.01).Td,Tdi,Tex,and Tin were significantly prolonged(P<0.05,P<0.01).Ad,Rd,RR,RE,RA,diaphragmatic muscular tension and endurance significantly decreased(P<0.05,P<0.01).The ratio of type Ⅰ and ⅡA diaphragmatic fibers significantly increased and type ⅡB significantly decreased(P<0.01).The activity of ATP decreased and the activity of SDH increased(P<0.01).The aforesaid indices were improved to different degrees in BJR groups,especially in the high dose BJR group and the medium dose BJR group(P<0.05,P<0.01).Conclusions BJR could significantly improve the diaphragmatic neural discharge and the diaphragmatic muscle function.Its efficacy was better than that of aminophyline.

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