口服与骨折局部应用淫羊藿对骨质疏松大鼠骨折愈合的影响

目的研究口服与骨折局部应用淫羊藿对骨质疏松大鼠骨折愈合的影响。方法取4月龄雌性清洁SD大鼠82只,体重210～250g,随机分成实验组75只,对照组7只。实验组切除双侧卵巢,对照组仅切除卵巢周围少许脂肪组织。术后10周骨密度及电镜证明实验组骨质疏松动物模型已制备成功。将实验组剩余的72只,再次手术制作右侧股骨远端松质骨骨折并随机分成3组,每组24只:磷酸钙骨水泥(calciumphosphate cement,CPC)治疗组(A组)、CPC-淫羊藿复合物治疗组(B组)及CPC加口服淫羊藿组(C组);分别在术后2、4、8、12周测定3组动物的血清碱性磷酸酶(alkaline phosphatase,ALP),进行股骨生物力学检测及组织切片观察。结果骨折术后2、4、8周时,3组大鼠血清ALP均有不同程度升高,但B组较A、C两组升高更为明显,差异有统计学意义(P<0.05)。12周时,B组ALP基本降至正常范围;C组和A组由于骨折仍未完全愈合,ALP虽有所下降,但仍维持在一个相对较高水平,与B组比较差异有统计学意义(P<0.05)。这与实验中组织学观察相一致,B组显示出更好的促成骨活性。B组在各个时间点的股骨生物力学检测均高于A、C两组(P<0.05)。结论局部应用淫羊藿能够促进骨质疏松骨折的愈合,相同剂量下比口服效用更显著。

英文摘要:

Objective To study the effects of administration or local application of epimedium on the fracture healing in osteoporosis rats.Methods Eighty-two 4-month old clean female rats,210-250 g,were randomly divided into the experimental group(n=75) and the control group(n=7).The bilateral ovaries were resected in the experimental group,while only little fat tissue around the ovary was resected in the control group.Ten weeks after operation the osteoporosis model was successfully established verified by bone densitometry and scanning electron microscopy(SEM).The femur fracture models were established in the rest 72 rats of the experimental group.They were randomly divided into 3 groups,24 in each group,i.e.,the calcium phosphate cement(CPC) group(Group A),the CPC-epimedium group(Group B),and the epimedium administration group(Group C).The serum alkaline phosphatase(ALP) levels of the 3 groups were determined 2,4,8,and 12 weeks after surgery.The vitodynamical test and observation of the histological section were performed.Results [WT9.75FZ] The serum ALP levels increased to some extent in the 3 groups 2,4,and 8 weeks after bone fracture surgery.But the increase was more obvious in Group B with statistical difference shown when compared with Group A and C(P<0.05).The ALP level in Group B decreased to the normal range till the 12th week.The bone fracture had not completely healed in Group C and A.Their ALP levels decreased to some extent,but were still maintained to a comparatively higher level,showing statistical difference when compared with that of Group B(P<0.05).These results were agreeable with the results of the histological observation.Better bone activity promoting results were shown in Group B.The vitodynamical test results of the femur of Group B were all higher than those of Group A and C at each time point(P<0.05).Conclusions Local application of epimedium could accelerate the fracture healing in osteoporosis rats.It showed better effects when compared with oral administration at the same dose.

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