化痰降气胶囊对COPD模型大鼠支气管上皮细胞中多药耐药相关蛋白1功能和表达的影响

Effects of Huatan Jiangqi Capsule on the Levels of Multi-drug Resistance-associated Protein 1 in the Bronchial Epithelial Cells of Model Rats with Chronic Obstructive Pulmonary Disease

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中文关键词: 化痰降气胶囊慢性阻塞性肺疾病多药耐药相关蛋白1 白三烯C4

英文关键词:Huatan Jiangqi Capsule chronic obstructive pulmonary disease multi-drug resistance-associated protein 1 leukotriene C4

基金项目:国家自然科学基金资助项目(No.81001592);教育部科学技术重点项目资助(No.210101);安徽高校省级自然科学研究重点项目资助(No.KJ2010A210)

作者	单位
汪电雷	安徽中医学院
张弦	安徽中医学院
陶秀华	安徽中医学院
张瑞	安徽中医学院
蔡莉	安徽中医学院
彭波	安徽中医学院第一附属医院呼吸科
李泽庚	安徽中医学院第一附属医院呼吸科

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中文摘要:

目的通过建立慢性阻塞性肺疾病(COPD)大鼠模型,观察化痰降气胶囊对支气管上皮细胞多药耐药相关蛋白1(MRP1)功能和表达的影响,探讨化痰降气胶囊治疗COPD的作用机制。方法雄性Wistar大鼠24只随机分为正常对照组、模型组和化痰降气胶囊组(简称化痰降气组)。除正常对照组外,其余各组采用烟、二氧化硫定量熏吸并复合木瓜蛋白酶雾化吸入法,建立COPD大鼠模型;观察化痰降气胶囊治疗后肺功能指标、支气管肺泡灌洗液细胞计数与分类、大鼠的肺组织病理学改变,并采用ELISA方法测定肺组织中白三烯C4(LTC4)的浓度及免疫组化测定肺支气管上皮MRP1的表达。结果 (1)模型组大鼠的肺顺应性(CL)、第0.3秒用力呼气容积(FEV0.3%)/用力肺活量(FVC)、呼气流量峰值、最大呼气中段流量较正常对照组显著降低(P<0.05)。化痰降气组上述肺功能指标较模型组明显升高(P<0.05)。(2)模型组气道炎症加重,且出现明显肺气肿;化痰降气组也出现了炎症和肺气肿,但程度较轻。(3)与正常对照组比较,模型组及化痰降气组肺组织中的LTC4均显著升高(P<0.01)。与模型组比较,化痰降气组中肺组织的LTC4显著下降(P<0.05)。(4)与正常对照组比较,模型组肺支气管上皮MRP1蛋白表达显著降低(P<0.01)。与模型组比较,化痰降气组肺支气管上皮MRP1蛋白表达显著增强(P<0.05)。结论化痰降气胶囊可能通过影响慢性阻塞性肺疾病状态下MRP1功能和表达,从而达到有效减轻COPD大鼠的气道炎症,延缓肺功能恶化等COPD发生和发展的目的。

英文摘要:

Objective To observe the effects of Huatan Jiangqi Capsule(HJC) on the expression levels and functions of multi-drug resistance-associated protein 1(MRP1) in the bronchial epithelial cells of chronic obstructive pulmonary disease(COPD) model rats,and to explore the mechanism of HJC for treating COPD.Methods Twenty-four male wistar rats were randomly divided into the normal control group,the model group,and HJC group.Except the normal control group,the COPD rat model was established in the rest groups using quantitative stimulation with tobacco,SO2,and caroid aerosol rebreathing method.The indices of the post-treatment lung functions,the cell counts of bronchoalveolar lavage fluid(BALF),the pathological features of the lung tissue were observed.The concentration of LTC4 in lung tissues was also examined by ELISA.The expression of MRP1 of the pulmonary tracheal epithelium was detected using immunohistochemical assay.Results(1) The pulmonary compliance,the forced expiratory volume at 0.3 second(FEV 0.3%)/the forced vital capacity(FVC),the peak expiratory flow,the maximum mid expiratory flow decreased more significantly in the model group than in the normal control group(P<0.05).The aforesaid pulmonary function indices obviously increased in the HJC group when compared with the model group(P<0.05).(2) The air inflammation was aggravated with obvious emphysema in the model group.The inflammation and emphysema occurred in the HJC group in a milder degree.(3) Compared with the normal control group,the levels of LTC4 significantly increased in the lung tissue of the model group and HJC group(P<0.01).Compared with the model group,the levels of LTC4 significantly decreased in the lung tissues of the HJC group(P<0.05).(4) Compared with the normal control group,the protein expression of the bronchial epithelial MRP1 significantly decreased in the model group(P<0.01).Compared with the model group,the protein expression of the bronchial epithelial MRP1 were significantly enhanced in the HJC group(P<0.05).Conclusion HJC could effectively alleviate the lung inflammation,postpone the occurrence and development of COPD possibly through effecting the functions and expressions of MRP1 in COPD rats.

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