益气养阴化瘀通络中药及其拆方对糖尿病大鼠模型nephrin蛋白的影响

Effects of Chinese Materia Medica of Qi Benefiting,Yin Nourishing,Stasis Removing,and Collaterals Dredging and Their Dissembled Recipes on Nephrin of Diabetes Mellitus Model Rats

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中文关键词: 益气养阴化瘀通络中药糖尿病尿白蛋白 nephrin

英文关键词:Chinese materia medica of qi benefiting,yin nourishing,stasis removing,and collaterals dredging diabetes mellitus urinary albumin nephrin

基金项目:河北省自然科学基金资助项目(No.C2008001074)

作者	单位
李黎莉	河北医科大学中西医结合研究所
陈志强	河北医科大学中西医结合研究所
王月华	河北医科大学中医院肾内科
张江华	河北医科大学中西医结合研究所
尹智炜	河北医科大学中西医结合研究所
李林林	河北医科大学中西医结合研究所
张雪云	河北医科大学中西医结合研究所
王凤丽	河北医科大学中西医结合研究所

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中文摘要:

目的观察益气养阴化瘀通络中药及其拆方对糖尿病(DM)大鼠模型nephrin蛋白的影响。方法高脂饲料联合小剂量STZ复制DM模型,设正常对照组(简称N组)、模型组(简称M组)均采用相应体积超纯水灌胃;益气养阴化瘀通络中药治疗组(简称YHT组),采用益气养阴化瘀通络中药,由黄芪、地黄、丹参、川芎各2袋,黄精、地龙、水蛭、全蝎各1袋中药配方颗粒配方,按1.0g/kg给药;益气养阴化瘀中药治疗组(简称YH组),采用益气养阴化瘀中药由黄芪、地黄、丹参、川芎各2袋,黄精1袋中药配方颗粒配方,按0.92g/kg给药;益气养阴通络中药治疗组(简称YT组),采用益气养阴通络中药,由黄芪、地黄各2袋,黄精、地龙、水蛭、全蝎各1袋中药配方颗粒配方,按0.79g/kg给药。定容至1mL/100g每日1次灌胃,共给药32周,监测大鼠体重。给药结束后,测定尿肌酐(UCr)、24h尿白蛋白(U-alb)和尿nephrin(U-nephrin)、空腹血糖(FBG)、糖化血红蛋白(HbA1c)、血清肌酐(SCr)、肾皮质nephrin(K-nephrin),肾组织切片Masson染色,观察病理学形态改变。结果 N组体重持续增加,造模后,M组和各给药组体重均明显下降,但各给药组体重下降较模型组轻,差异有统计学意义(P<0.01)。与N组比较,M组U-alb和U-nephrin显著增加(P<0.01),并且两者呈正相关(r=0.941,P=0.017),K-nephrin显著降低,K-nephrin和U-alb呈负相关(r=-0.987,P=0.002),FBG、内生肌酐清除率(CCr)和HbA1c显著升高(P<0.01),光镜检查可见肾小球明显肥大,细胞外基质明显增加。与M组比较,除YT组FBG和HbA1c外相应指标均有明显改善(P<0.01)。给药组间比较,YHT组改善CCr显著优于YH组和YT组,差异有统计学意义(P<0.01),其余指标各给药组间比较,差异均无统计学意义(P>0.05)。各给药组肾小球肥大不如M组明显,细胞外基质增加不明显。结论益气养阴化瘀通络中药及其拆方对DM大鼠模型有肾保护作用,减少nephrin的丢失,从而减少U-alb,可能是其作用途径之一。

英文摘要:

Objective To observe the effects of Chinese materia medica(CMM) of qi benefiting,yin nourishing,stasis removing,and collaterals dredging(QBYNSRCD) and their dissembled recipes on nephrin of diabetes mellitus(DM) model rats.Methods The DM model was induced by high fat diet combined with low dose STZ.Rats in the normal control group(abbreviated as Group N) and the model group(abbreviated as Group M) were administered with ultrapure water at corresponding volume by gastrogavage.Rats in the CMM of QBYNSRCD treatment group(abbreviated as Group YHT) were administered with CMM of QBYNSRCD,composed of milkvetch root,rehmannia root,danshen root,chuanxiong(2 packages each),solomonseal,earth worm,leech,and scorpion(1 package each),which was administered at 1.0 g/kg.Rats in the CMM of qi benefiting,yin nourishing,and stasis removing(QBYNSR) treatment group(abbreviated as Group YT) were administered with CMM of QBYNSR,composed of milkvetch root,rehmannia root,danshen root,chuanxiong(2 packages each),and solomonseal(1 package each),which was administered at 0.92 g/kg.Rats in the CMM of qi benefiting,yin nourishing,and collaterals dredging(QBYNCD) treatment group(abbreviated as Group YT) were administered with CMM of QBYNCD,composed of milkvetch root,rehmannia root(2 packages each),solomonseal,earth worm,leech,and scorpion(1 package each),which was administered at 0.79 g/kg.The volume was set to 1 mL/100 g,once daily by gastrogavage,for total 32 weeks.Rats’ body weight was measured.By the end of medication,urinary creatinine(UCr),24-h urinary albumin(U-alb),and urinary nephrin(U-nephrin),fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c),serum creatinine(SCr),and nephrin of kidney tissues homogenate(K-nephrin) were detected.The renal tissue sections were stained with Masson.The pathomorphological changes were observed.Results The body weight of rats in Group N increased gradually.After modeling,the body weight of rats in Group M and all medicated groups obviously decreased.Compared with Group M,the decreased body weight was not obvious in all medicated groups,still showing statistical difference(P<0.01).Compared with Group N,U-alb and U-nephrin in Group M significantly increased(P<0.01) in a positive linear correlation(r=0.941,P=0.017).K-nephrin significantly decreased,and K-nephrin had a negative linear correlation with U-alb(r=-0.987,P=0.002).FBG,CCr,and HbA1c significantly increased(P<0.01).Glomeruli were obviously enlarged under light microscope,with obviously increased extracellular matrix accumulation.Compared with Group M,corresponding indices were obviously improved(P<0.01) except FBG and HbA1c in Group YT.As for inter-group comparison among all medicated groups,the improvement of CCr was the best in Group YHT with statistical difference shown(P<0.01).There was no statistical difference for the rest indices(P>0.05).When compared with Group M,the hypertrophy of glomerulus was not so obvious in all medicated groups.Neither was extracellular matrix accumulation.Conclusions CMM of QBYNSRCD and dissembled recipes showed renal protection on DM model rats.One of its action pathways might be reducing the loss of nephrin,thus reducing U-alb.

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