| 黄宝英,曹罗元,富显果,刘金发,杨菁,陈刚.丹参酮ⅡA对高糖诱导肾小管上皮细胞转分化中Wnt/β-catenin信号途径的影响[J].中国中西医结合杂志,2012,32(7):965-969 |
| 丹参酮ⅡA对高糖诱导肾小管上皮细胞转分化中Wnt/β-catenin信号途径的影响 |
| Effects of Tanshinone ⅡA on Wnt/β-catenin Signaling Pathway of High Glucose Induced Renal Tubular Epithelial Cell Transdifferentiation |
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| DOI： |
| 中文关键词: E-钙粘蛋白 β-连环蛋白 α-平滑肌肌动蛋白 上皮细胞转分化 丹参酮ⅡA |
| 英文关键词:E-cadherin β-catenin α-smooth muscle actin epithelial cell transdifferentiation tanshinone ⅡA |
| 基金项目:福建省自然科学基金科技项目(No.2012J01435);福建省宁德市卫生类科技计划项目(No.20100144) |
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| 中文摘要: |
| 目的探究高糖诱导肾小管上皮细胞转分化过程中Wnt/β-catenin信号途径的表达及丹参酮ⅡA对其影响。方法将人近端肾小管上皮细胞(HK-2),分为正常糖组(NG组)、高糖组(HG组)、高糖+丹参酮ⅡA干预组(HG+T组)。采用免疫细胞化学观察β-catenin表达情况;Western印迹检测β-catenin、上皮细胞标志性蛋白E-钙粘蛋白(E-cadherin)、间充质细胞标志性蛋白α-SMA的表达;RT-PCR检测β-cate-nin、E-cadherin mRNA表达水平。结果与NG组比较,HG组β-catenin蛋白及mRNA表达显著增强(P<0.01),E-cadherin表达显著降低(P<0.01),β-catenin蛋白在胞浆与胞核表达增强。终浓度为100μmol/L的丹参酮ⅡA可显著减少β-catenin的异位表达;在该浓度下,β-catenin在胞核蛋白及mRNA的表达量显著下降,而E-cadherin蛋白及mRNA的表达水平上升,同时间充质细胞标志性蛋白α-SMA的表达明显下降。结论 Wnt/β-catenin信号途径参与了高糖诱导肾小管上皮细胞转分化过程,丹参酮ⅡA可能通过下调Wnt/β-catenin信号途径的活性而抑制转分化过程,进而发挥保护肾脏的作用。 |
| 英文摘要: |
| Objective To observe the expressions of Wnt/β-catenin and the effects of tanshinone ⅡA(TⅡA) on Wnt/β-catenin signaling pathway in high glucose induced renal tubular epithelial cell transdifferentiation.Methods Human kidney proximal tubular epithelial cells(HK-2) were divided into three groups,i.e.,the normal glucose group,the high glucose group,and the high glucose plus tanshinone ⅡA group.The expression of β-catenin was observed using immunocytochemical staining.The protein expression of β-catenin,E-cadherin,and α-smooth muscle actin(α-SMA) were detected by Western blot.The mRNA levels of β-catenin and E-cadherin were detected by RT-PCR.Results Compared with the normal glucose group,both the protein and the mRNA expressions of β-catenin were significantly enhanced(P<0.01),the expression of E-cadherin significantly decreased(P<0.01),the expression of β-catenin increased in the cytoplasm and nucleus in the high glucose group.TⅡA at the final concentration of 100 μmol/L significantly reduced the ectopic expression of β-catenin.At that concentration,the protein and mRNA expressions of β-catenin in the nucleus significantly decreased,while the protein and mRNA expressions of E-cadherin were up-regulated.Meanwhile,the expression of α-SMA obviously decreased.Conclusions Wnt/β-catenin signaling pathway participated in the high glucose induced renal tubular epithelial cell transdifferentiation.TⅡA inhibited the transdifferentiation process possibly through down-regulating the activities of Wnt/β-catenin signaling pathway,thus further playing a role in renal protection. |
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