慢性无症状HBV携带者阴虚体质与人类白细胞抗原-DRB1、DQA1基因多态性的联系

作者	单位
过建春	杭州市第六人民医院中西医结合科
肖丽娜	浙江省桐庐县人民医院感染科
荀运浩	杭州市第六人民医院中西医结合科
王宇芳	杭州市第六人民医院中西医结合科
李春青	杭州市第六人民医院中西医结合科
石伟珍	杭州市第六人民医院中西医结合科
施军平	杭州市第六人民医院中西医结合科

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中文摘要:

目的探讨慢性无症状HBV携带者(chronic asymptomatic HBV carrier,ASC)阴虚体质与人类白细胞抗原(human leukocyte antigen,HLA)-DRB1、HLA-DQA1基因多态性的内在联系。方法将105例ASC患者进行中医体质分型及分组:阴虚质组47例和非阴虚质组58例。采用聚合酶链反应-序列特异性引物(PCR-SSP)技术测定HLA-DRB1、HLA-DQA1等位基因型。结果阴虚质组HLA-DRB1*09、HLA-DQA1*0301的基因频率明显低于非阴虚质组(分别为12.1%vs27.8%,19.1%vs39.7%),差异有统计学意义(P<0.01);HLA-DRB1*11、HLA-DQA1*0501的基因频率明显高于非阴虚质组(分别为12.1%vs4.3%,28.7%vs9.5%),差异有统计学意义(P<0.05,P<0.01)。结论 HLA-DRB1*09和HLA-DQA1*0301可能是ASC非阴虚体质患者的分子基础;HLA-DRB1*11和HLA-DQA1*0501可能是ASC阴虚体质患者的分子基础。

英文摘要:

Objective To study on the correlation between chronic asymptomatic HBV carriers(ASC) of yin asthenia constitution and genotypes of HLA-DRB1 and HLA DQA1 alleles.Methods Totally 105 ASC were assigned to two groups according to their constitutions,i.e.,the yin asthenia group(47 cases) and the non-yin asthenia group(58 cases).The genotypes of HLA-DRB1 and HLA DQA1 alleles were determined using PCR-SSP.Results The gene frequency of HLA-DRB1*09 allele and HLA-DQA1*0301 allele(being 12.1% and 19.1%) were obviously lower in the yin asthenia group than in the non-yin asthenia group(being 27.8% and 39.7%,P<0.05).The gene frequency of HLA-DRB1*11 allele and HLA-DQA1*0501 allele were obviously higher in the yin asthenia group(being 12.1% and 28.7%) than in the non-yin asthenia group(4.3% and 9.5%),showing statistical difference(P<0.05,P<0.01).Conclusions HLA-DRB1*09 allele and HLA-DQA1*0301 allele might be the molecular bases for non-yin asthenia patients with ASC.HLA-DRB1*11 allele and HLA-DQA1*0501 allele might be the molecular bases for yin asthenia patients with ASC.

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