银杏叶提取物对衰老大鼠前额叶皮层和海马炎性细胞因子及胶质细胞超微结构的影响

Effects of Ginkgo biloba Extract 50 on Inflammatory Cytokines and Glia Cell Ultrastructures in the Prefrontal Cortex and Hippocampus of Aging Rats

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中文关键词: 衰老前额叶皮层海马细胞因子胶质细胞银杏叶提取物

英文关键词:aging the prefrontal cortex the hippocampus cytokine glia cell Ginkgo biloba extract 50

基金项目:上海市自然科学基金资助项目(No.09ZR1432100);上海市教委重点学科资助项目(No.J50301);上海市教委预算内科研项目(No.2010JW04)

作者	单位
贺改英	上海中医药大学生理教研室
张志雄	上海中医药大学生理教研室
徐颖	上海中医药大学生理教研室
董献文	上海中医药大学生理教研室
王星禹	上海中医药大学生理教研室

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中文摘要:

目的探讨银杏叶提取物(Ginkgo biloba extract 50,GBE50)对衰老模型大鼠前额叶皮层和海马炎性细胞因子以及胶质细胞损伤的作用及可能的机制。方法 45只雄性SD大鼠随机分为正常对照组(12只)、模型组(11只)、GBE50低剂量组(10只)和GBE50高剂量组(12只)。采用D-半乳糖腹腔注射42天的方法建立衰老大鼠模型;造模第22天开始,GBE50低、高剂量组分别给予GBE5075、150mg/(kg·d)灌胃给药,持续21天。用药结束后采用放射免疫分析法和实时荧光定量聚合酶链反应检测各组大鼠前额叶皮层和海马IL-1β、IL-6和TNF-α蛋白含量和mRNA表达;透射电镜观察海马CA1区胶质细胞的超微结构。结果与正常对照组比较,模型组大鼠前额叶皮层和海马IL-1β、TNF-α蛋白含量及mRNA表达均明显升高(P<0.05,P<0.01),海马IL-6蛋白含量明显降低(P<0.01)。与模型组比较,GBE50低、高剂量组前额叶皮层和海马IL-1β蛋白含量及mRNA表达明显下调;GBE50低、高剂量组前额叶皮层和低剂量组海马TNF-α蛋白含量明显下调(P<0.05,P<0.01);GBE50低剂量组前额叶皮层和GBE50高剂量组海马IL-6蛋白含量明显上调,GBE50低、高剂量组前额叶皮层IL-6mRNA表达明显升高(P<0.05,P<0.01)。GBE50低、高剂量对大鼠海马CA1区胶质细胞超微结构的损伤具有明显恢复作用。结论 GBE50对衰老大鼠神经炎症反应具有抑制作用,其机制可能与其对前额叶皮层和海马细胞因子、胶质细胞超微结构有不同程度的调节作用有关。

英文摘要:

Objective To study the effects of Ginkgo biloba extract 50(GBE50) on inflammatory cytokines and glia cell injury in the prefrontal cortex and hippocampus of aging rats and its probable mechanism.Methods Totally 45 male SD rats were randomly divided into 4 groups,i.e.the normal control group(n=12),the model group(n=11),the low dose GBE50 group(n=10),and the high dose GBE50 group(n=12).The aging rat model was intraperitoneally injected with D-galactose to establish the aging model for 42 days.Starting from the 22nd day of modeling,rats in the low dose GBE50 group and the high dose GBE50 group were administered by gastrogavage with 75 mg/kg and 150 mg/kg respectively.The protein contents and mRNA expressions of IL-1β,IL-6,and TNF-α in the prefrontal cortex and hippocampus of rats were detected by radioimmunoassay and Real-time fluorescence quantitative PCR assay respectively.The ultrastructural changes of glia cells in the hippocampal CA1 region were observed by transmission electron microscope.Results The protein contents and mRNA expressions of IL-1β and TNF-α in the prefrontal cortex and the hippocampus of aging rats obviously increased when compared with the normal control group(P<0.05,P<0.01).The content of IL-6 in the hippocampus of aging rats obviously decreased(P<0.01).Compared with the model group,the protein content and mRNA expression of IL-1β in the prefrontal cortex and the hippocampus were obviously down-regulated in the low and high dose GBE50 groups.The content of TNF-α in the prefrontal cortex was obviously down-regulated in the low and high dose GBE50 groups,the content of TNF-α in the hippocampus was obviously down-regulated in the low dose GBE50 group(P<0.05,P<0.01).The content of IL-6 in the prefrontal cortex of the low dose GBE50 group was up-regulated.The content of IL-6 in the hippocampus of the high dose GBE50 group was also up-regulated.The mRNA expression of IL-6 in the prefrontal cortex of the low and high dose GBE50 groups obviously increased(P<0.05,P<0.01).Low and high dose GBE50 showed obvious recovery on the ultrastructural damage of glia cells in the hippocampal CA1 region.Conclusions GBE50 showed inhibitive effects on the inflammatory reaction of nerves of aging rats.Its mechanism might be possibly correlated with its regulatory effects on the cytokines in the prefrontal cortex and the hippocampus,as well as the ultrastructures of glia cells in the prefrontal cortex and hippocampus to some degree.

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