| 魏明刚,张玲,倪莉,李凤玲,俞立强,孙美娟,熊佩华.加味当归补血汤对阿霉素肾病大鼠肾脏足细胞保护机制的研究[J].中国中西医结合杂志,2012,32(8):1077-1082 |
| 加味当归补血汤对阿霉素肾病大鼠肾脏足细胞保护机制的研究 |
| Protection Mechanisms of Modified Danggui Buxue Decoction for Podocytes in Adriamycin-induced Nephropathy Rats |
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| DOI： |
| 中文关键词: 加味当归补血汤 阿霉素肾病 足细胞 |
| 英文关键词:Modified Danggui Buxue Decoction adriamycin-induced nephropathy podocyte |
| 基金项目:苏州市科学技术局应用基础研究计划(No.SYS201016);苏州大学青年教师自然科学基金(No.Q3122937) |
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| 中文摘要: |
| 目的利用阿霉素肾病模型研究加味当归补血汤对肾小球足细胞作用的影响。方法实验动物分为正常组、模型组、贝那普利组和加味当归补血汤组。分别在造模第7、28、42、56天留取尿液标本,观察尿白蛋白定量的动态变化;取肾组织进行光镜、电镜和足细胞表达蛋白nephrin、podocin的免疫荧光检测,并进一步应用分子生物学(RT-PCR及Wetern blot)的方法对肾组织中足细胞裂孔膜表达蛋白的表达情况进行分析。结果 (1)尿白蛋白:各治疗组治疗7天尿白蛋白减少不明显,但在28、42、56天表现为非常明显的下降,贝那普利组和加味当归补血汤组与模型组比较,差异均有统计学意义(P<0.05),而且加味当归补血汤最为明显;(2)肾脏组织和足细胞的影响:光镜和电镜观察结果显示加味当归补血汤治疗组与贝那普利治疗组和模型组比较,系膜细胞增生情况、肾小管-间质病变情况、足细胞融合和足细胞表达蛋白nephrin、podo-cin的表达情况均较轻,尿白蛋白定量相对模型组明显减少的情况下仍然存在与肾脏纤维化相关的肾脏病理进展,说明肾脏病变程度相比模型组较轻但不是逆转;(3)治疗第56天肾脏组织足细胞RT-PCR及Weternblot检测nephrin、podocin表达;各治疗组与模型组比较,差异有统计学意义(P<0.05)。结论加味当归补血汤对于阿霉素肾病模型具有治疗作用,作用主要是通过减少尿白蛋白,抑制系膜细胞增生和减轻肾小管-间质损伤,保护足细胞裂孔膜结构的完整性。 |
| 英文摘要: |
| Objective To study the effects of Modified Danggui Buxue Decoction(MDBD) on podocytes in adriamycin-induced nephropathy(DN) model rats.Methods SD rats were divided into four groups,i.e.,the normal control group,the model group,the benazepril group,and the MDBD group.On the 7th,28th,42nd,and 56th day of modeling,the urine sample was collected to examine the dynamic changes of urinary albumin quantitation.The renal tissue was processed for the examinations under light microscope and electron microscope.The immunofluorescence of nephrin and podocin were detected.The expressions of the slit membrane expression protein in the renal tissue were further analyzed using RT-PCR and Western blot.Results(1) Urinary protein(UP):The UP did not obviously decrease in each treatment group on the 7th day,but it decreased so markedly on the 28th,42nd,and 56th day.There was statistical difference in UP of the benazepril group and the MDBD group when compared with that of the model group(P<0.05).The decrease was most obvious in the MDBD group.(2) Effects on the podocytes and the renal tissue:Results under light microscope and electron microscope showed,when compared with the model group,the proliferation of mesangial cells,the renal tubule-interstitial lesion,the podocyte fusion,and the expressions of nephrin and podocin were milder,and the urinary albumin quantitation was more obviously reduced in the benazepril group and the MDBD group.But the renal fibrosis correlated renal pathological progress also existed,indicating the renal lesion degree was milder but could not be reversed.(3) Results of RT-PCR and Western blot:Statistical difference existed in the expressions of nephrin and podocin between the benazepril group and the MDBD group on the 56th day,when compared with the model group(P<0.05).Conclusion MDBD showed therapeutic effects on adriamycin-induced nephropathy model rats.Its actions could be achieved through reducing albuminuria,inhibiting the proliferation of mesangial cells,attenuating the renal tubule-interstitial lesion,and protecting the integrity of the slit membrane structure. |
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