降糖消脂片对转基因小鼠降糖降脂作用的实验研究

Experimental Study of Jiangtang Xiaozhi Tablet on Decreasing the Levels of Blood Glucose and Serum Lipids in Transgenic Mice with Diabetes Mellitus

免费下载全文查看/发表评论下载PDF阅读器

DOI：

中文关键词: 降糖消脂片转基因小鼠胰岛素瘦素

英文关键词:Jiangtang Xiaozhi Tablet transgenic mouse insulin leptin

基金项目:综合性中药新药研究开发技术大平台资助项目(No.2009ZX09301-005-007);重大新药创制——中药医院制剂新药研发项目(No.2010ZX09102-213)

作者	单位
葛争艳	中国中医科学院西苑医院实验研究中心
金龙	中国中医科学院西苑医院实验研究中心
郭宇洁	中国中医科学院西苑医院实验研究中心
杨斌	中国中医科学院西苑医院实验研究中心
董小霞	中国中医科学院西苑医院实验研究中心
李宏坤	中国中医科学院西苑医院实验研究中心
任烨	中国中医科学院西苑医院实验研究中心
刘建勋	中国中医科学院西苑医院实验研究中心

摘要点击次数: 2078

全文下载次数: 0

中文摘要:

目的建立糖尿病肥胖小鼠模型,观察降糖消脂片对其血糖、血脂等指标的影响。方法采用KK-Ay转基因小鼠,高脂饲料喂养,造成糖尿病肥胖小鼠模型。对照组为同龄C57小鼠,成模的KK-Ay小鼠分为:模型组、吡格咧酮组(8mg/kg)、降糖消脂片大剂量组(简称降糖消脂大组,10g/kg)、降糖消脂片中剂量组(简称降糖消脂中组,5g/kg)、降糖消脂片小剂量组(简称降糖消脂小组,2.5g/kg),每组11只。灌胃给药8周。每周称体重及摄食量,隔周测血糖(FBG)。药后8周测FBG、总胆固醇(TC)、甘油三酯(TG)、胰岛素(INS)、瘦素(Lep);取肝脏、脂肪称重,计算其脏器指数;并对肝脏的脂肪变性进行分级、计数胰岛数目及脂肪细胞数。结果与模型组比较,降糖消脂大、中、小组体重明显减轻、摄食量明显下降(P<0.05,P<0.01);降糖消脂大组从第6周开始,FBG明显下降(P<0.01);连续灌胃给药8周后,降糖消脂大、中、小组的TG、INS明显下降(P<0.05,P<0.01);降糖消脂大组Lep有所下降(P<0.05);降糖消脂大、中、小组肝脏和脂肪指数明显降低、胰岛数目明显增多(P<0.05,P<0.01);降糖消脂大、中组的脂肪细胞数目明显减少(P<0.05,P<0.01);降糖消脂大组肝脏的脂肪变性明显改善(P<0.05)。结论降糖消脂片对KK-Ay糖尿病肥胖小鼠有明显的降糖、降脂作用。

英文摘要:

Objective To observe the effects of Jiangtang Xiaozhi Tablet(JTXZT) on decreasing the levels of blood glucose and serum lipids in KK-Ay transgenic model mice with diabetes mellitus(DM).Methods KK-Ay transgenic mice were fed with high fat diet to induce DM obesity model.The C57 mice of the same age were recruited as the control group.Then the modeled KK-Ay mice were divided into 5 groups,i.e.,the model group,the Pioglitazone Hydrochloride Tablet treatment group(8 mg/kg,as the positive control group),and the high dose JTXZT group(10 g/kg),the middle dose JTXZT group(5 g/kg),and the low dose JTXZT group(2.5 g/kg),11 in each group.Eight weeks was taken as one therapeutic course.All medicines were given by gastrogavage.The food intake and body weight were measured every week.The blood glucose level was determined once every 2 weeks.The levels of fasting blood glucose(FBG),serum lipids(TC and TG),insulin(INS),and leptin(Lep) were assayed 8 weeks after medication.The weight of liver and fat were weighed to calculate their indices.Then the adipose denaturalization of the liver was classified.The numbers of the pancreatic islet and adipocytes were respectively counted.Results Compared with the model group,the food intake and the body weight obviously decreased in the 3 JTXZT groups(P<0.05,P<0.01).From the 6th week,the FBG level obviously decreased in the high dose JTXZT group(P<0.01).After eight successive weeks of intragastric administration,the levels of TG and INS obviously decreased in the 3 JTXZT groups(P<0.05,P<0.01).The Lep level somewhat decreased in the high dose JTXZT group(P<0.05).The indices of the liver and fat obviously decreased and the number of the pancreatic islet obviously increased in the 3 JTXZT groups(P<0.05,P<0.01).The number of adipocytes obviously decreased in the high and middle dose JTXZT groups(P<0.05,P<0.01).The liver fatty degeneration was obviously improved in the high dose JTXZT group(P<0.05).Conclusion JTXZT had obvious effects on decreasing the levels of blood glucose and serum lipids in KK-Ay transgenic model mice with DM obesity.

关闭