散结镇痛胶囊和达那唑对大鼠子宫内膜异位症治疗效果的比较

作者	单位
邹杰	中国人民解放军总医院妇产科
关铮	中国人民解放军总医院妇产科
张唯一	中国人民解放军总医院妇产科
刘慧	中国人民解放军总医院妇产科
周春艳	中国人民解放军总医院实验动物中心
李亚里	中国人民解放军总医院妇产科
萧伟	江苏康缘药业股份有限公司

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中文摘要:

目的比较散结镇痛胶囊和达那唑对大鼠子宫内膜异位症(endometriosis,EMT)模型的治疗效果。方法选取48只成熟雌性Lewis大鼠,12只为空白对照组,其余36只于动情期建立大鼠EMT模型后随机分为3组,模型对照组、散结镇痛胶囊(散结)治疗组、达那唑治疗组,每组12只。4周后第2次开腹测量病灶大小。模型对照组给予生理盐水1mL/d,散结治疗组给予散结镇痛胶囊86.4mg/d,达那唑治疗组给予达那唑7.2mg/d,治疗持续4周。治疗结束后,行第3次开腹,测量病灶大小、免疫组化法检测病灶增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和以末端转移酶介导的缺口末端标记法(terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling,TUNEL)检测细胞凋亡率,ELISA法检测大鼠腹水和血清前列腺素E2(prostaglandin E2,PGE2)浓度。结果治疗前后病灶体积变化散结治疗组(-23.27±18.18)和达那唑治疗组(-12.28±10.04)与模型对照组(13.97±7.54)比较,差异有统计学意义(P<0.01)。两治疗组较模型对照组异位病灶PCNA表达明显下降而TUNEL阳性表达率增加(P<0.05,P<0.01),散结治疗组较达那唑治疗组异位病灶PCNA表达明显下降而TUNEL阳性表达率增加(P<0.01)。两治疗组较模型对照组腹水和血清中PGE2浓度明显降低(P<0.01),散结治疗组与达那唑治疗组比较腹水和血清中PGE2浓度明显降低(P<0.01)。结论散结镇痛胶囊和达那唑均能抑制大鼠EMT病灶生长,散结镇痛胶囊效果优于达那唑,为治疗EMT的有效药物。

英文摘要:

Objective To compare the effects of Sanjie Zhentong Capsule(SZC) and danazol on rats with endometriosis(EMT).Methods Totally 48 adult female Lewis rats were selected,12 as the blank control group,and the rest 36 rats in the estrus cycle were used to establish the EMT model.After modeling they were randomly divided into 3 groups,i.e.,the model control group,the SZC treatment group,and the danazol treatment group,12 in each group.Four weeks later the focus was measured by a second laparotomy.The normal saline at 1 mL/day was administered to rats in the model control group,SZC at 86.4 mg/day to those in the SZC treatment group,and danazol at 7.2 mg/day to those in the danazol treatment group.All the treatment lasted for 4 weeks.At the end of the treatment,a third laparotomy was performed to measure the size of focus.The expression of proliferating cell nuclear antigen(PCNA) was detected using immunohistochemical assay.The cell apoptosis rate was detected using terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling(TUNEL).The concentration of prostaglandin E2(PGE2) in the peritoneal fluid and the serum were detected using ELISA.Results There was statistical difference in the change of the focus volume between the SZC treatment group(-23.27±18.18) and the danazol treatment group(-12.28±10.04) and the model control group(13.97±7.54,P<0.01).The expression of ectopic PCNA significantly decreased and the positive expression rate of TUNEL obviously increased in the two treatment groups when compared with the model control group(P<0.05,P<0.01).The expression of ectopic PCNA decreased and the positive expression rate of TUNEL increased more obviously in the SZC treatment group than in the danazol treatment group(P<0.01).The concentration of PGE2 in the peritoneal fluid and the serum was significantly lower in the two treatment group when compared with the model control group(P<0.01).The concentration of PGE2 in the peritoneal fluid and the serum was significantly lower in the SZC treatment group than in the danazol treatment group(P<0.01).Conclusions SZC and danazol both could inhibit the focus growth in EMT rats.SZC showed better effects.It was an effective drug for treating EMT.

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