不同证型慢性胃炎患者外周血淋巴细胞HSP70、NF-~κB的表达研究

作者	单位
胡玲	广州中医药大学脾胃研究所
郑晓凤	澳门光辉医疗康复中心
鄢雪辉	浙江省金华市中医院消化内科
梁嘉恺	广州中医药大学脾胃研究所
古文莉	广州中医药大学第一附属医院内镜室
劳绍贤	广州中医药大学脾胃研究所

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中文摘要:

目的探讨脾胃湿热、脾气虚证外周血淋巴细胞热休克蛋白70(HSP70)和核因子-κB(NF-κB)的表达及其与Hp感染的关系。方法选择86例2008年6月—2009年2月就诊于广州中医药大学第一附属医院脾胃科门诊的慢性胃炎患者。其中脾胃湿热证67例(湿重于热30例,湿热并重27例,热重于湿10例)、脾气虚证19例,另选择广州中医药大学健康教职工和学生志愿者12名作为对照组。采集各组外周血,采用安速幽门螺旋杆菌(Hp)血清快速法检测Hp感染;采用流式细胞仪检测HSP70、NF-κB表达。结果脾胃湿热与脾气虚证Hp感染率分别为37.31%和36.84%(P>0.05)。与对照组比较,脾胃湿热证热重于湿组淋巴细胞HSP70表达降低,脾胃湿热证热重于湿组及脾气虚证组NF-κB表达升高(P<0.05)。脾胃湿热、脾气虚证Hp阳性组NF-κB表达均高于对照组(P<0.05);脾气虚证Hp阳性组HSP70表达高于同证型Hp阴性组(P<0.05)。此外,HSP70、NF-κB表达与Hp感染相关系数分别为-0.023和0.027(P>0.05)。结论慢性胃炎患者外周血淋巴细胞NF-κB在脾胃湿热证热重于湿、脾气虚证表达的升高可能反映了中医理论中"内邪"的致病作用;HSP70在脾气虚证HP阳性表达的升高,及在脾胃湿热证热重于湿表达的降低可能反映了"正气抗邪"、"邪盛正虚"的作用;Hp可能并非引起脾胃湿热证、脾气虚证发生的唯一"外邪"因素。

英文摘要:

Objective To study the expressions of heat shock protein70(HSP 70) and nuclear factor-kappa B(NF-κB) in the peripheral blood lymphocyte of chronic gastritis(CG) patients of Pi-Wei hygropyrexia syndrome(PWHS) and Pi-qi deficiency syndrome(PQDS),and to explore their correlation with Helicobacter pylori(Hp) infection.Methods Recruited were totally 86 CG patients who visited at the clinics of gastroenterology,First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,including 67 patients of PWHS(30 of predominant-dampness,30 of equal dampness and heat,and 30 of predominant-heat) and 19 patients of PQDS.Another 12 volunteers from healthy employees and students of Guangzhou University of Traditional Chinese Medicine were recruited as the control group.Their peripheral blood was collected.The Hp infection was detected using ASSURE Hp rapid test.The expressions of HSP 70 and NF-κB in the peripheral blood lymphocyte were detected using flow cytometry.Results The Hp infection rate was 37.31% in the GS patients of PWHS and 36.84% in the GS patients of PQDS(P>0.05).Compared with the control group,the expression of HSP 70 decreased in the PWHS predominant-heat group,and the expression of NF-κB increased in the PWHS predominant-heat group and the PQDS group(P<0.05).The expression of NF-κB were higher in the positive Hp infection patients of PWHS and PQDS than in the control group(P<0.05).The expression of HSP 70 was higher in the positive Hp infection patients of PQDS than in the negative Hp infection patients of PQDS(P<0.05).Besides,the coefficient correlation was-0.023 between HSP 70 and Hp infection,and 0.027 between NF-κB and Hp infection(P>0.05).Conclusions The increased expression of NF-κB in the peripheral blood lymphocyte of CG patients of PWHS and PQDS might reflect the pathogenic roles of "inner evil" in Chinese medicine theories.The increased expression of HSP 70 in CG patients of PQDS and decreased expression of HSP 70 in CG patients of PWHS might reflect "vital qi fighting against evils" and "exuberance evils and feeble vital qi" in the body.Hp infection might not be the only factor resulting in the occurrence of PWHS or PQDS.

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