| 景钊,邹海洲,许芳,邹长林.温郁金提取物对食管癌TE-1细胞增殖的抑制作用[J].中国中西医结合杂志,2012,32(9):1219-1222 |
| 温郁金提取物对食管癌TE-1细胞增殖的抑制作用 |
| The Molecular Mechanisms of Curcuma Wenyujin Extract-mediated Inhibitory Effects on Human Esophageal Carcinoma Cells in Vitro |
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| DOI： |
| 中文关键词: 温郁金 食管癌 TE-1细胞 基因芯片 |
| 英文关键词:Curcuma Wenyujin esophageal cancer cell line TE-1 cDNA microarray |
| 基金项目:浙江省中医药资助项目(No.2008CB051) |
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| 中文摘要: |
| 目的研究温郁金提取物对食管癌TE-1细胞增殖的抑制作用。方法采用超临界CO2萃取法提取温郁金成分。将TE-1细胞贴壁后分成4组:1组加入100μL含0.1%DMSO的RMPI-1640培养基,作为对照组;另外3组分别加入浓度为25、50、100mg/L温郁金提取物完全培养液各100μL,分别作为温郁金低、中、高剂量组。培养48h后采用四甲基偶氮唑盐(MTT)比色法测定各组TE-1细胞生长抑制率。应用基因芯片技术检测各组TE-1细胞基因表达谱的变化。对差异表达基因进行基因本体(gene ontology,GO)功能分类。结果与对照组比较,温郁金各剂量组生长抑制率均升高,差异有统计学意义(P<0.05)。温郁金各剂量组TE-1细胞生长抑制率随着剂量升高而增加,差异均有统计学意义(P<0.05)。通过基因芯片筛选出温郁金提取物作用前后88个基因表达水平发生改变且有统计学意义,包括22个基因表达下调,66个基因表达上调。基因GO分类提示差异表达基因主要涉及信号传导(6个)、细胞周期(8个)、凋亡(14个)或分化调节(10个)等。结论温郁金提取物对人食管癌TE-1细胞生长有抑制作用,其抗肿瘤作用可能与调控多层次的基因表达改变有关。 |
| 英文摘要: |
| Objective To study the molecular mechanisms of Curcuma Wenyujin extract-mediated inhibitory effects on human esophageal carcinoma cells.Methods The Curcuma Wenyujin extract was obtained by supercritical carbon dioxide extraction.TE-1 cells were divided into 4 groups after adherence.100 μL RMPI-1640 culture medium containing 0.1% DMSO was added in Group 1 as the control group.100 μL 25,50,and 100 mg/L Curcuma Wenyujin extract complete culture medium was respectively added in the rest 3 groups as the low,middle,and high dose Curcuma Wenyujin extract groups.The effects of different doses of Curcuma Wenyujin extract(25,50,and 100 mg/L)on the proliferation of human esophageal carcinoma cell line TE-1 in vitro were analyzed by MTT assay.The gene expression profile was identified by cDNA microarrays in esophageal carcinoma TE-1 cells exposed to Curcuma Wenyujin extract for 48 h.The differential expression genes were further analyzed by Gene Ontology function analysis.Results Compared with the control group,MTT results showed that Curcuma Wenyujin extract significantly inhibited the proliferation of TE-1 cells in a dose-dependent manner(P<0.05).The expression level of 88 genes changed with significance,including 66 up-regulation genes and 22 down-regulation genes.Gene Ontology analysis indicated the genes coding for proteins was involved in signal transduction(6),cell cycle(8),apoptosis(14),and cell differentiation(10).Conclusions The Curcuma Wenyujin extract could inhibit the growth of human esophageal carcinoma cell line TE-1 in vitro.The molecular mechanisms might be associated with regulating genes expressions at multi-levels. |
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