补肾调经方对人卵泡壁颗粒细胞BMP/Smads表达的影响

作者	单位	E-mail
梁莹，赵胜男，常秀峰
杜惠兰	河北医科大学中西医结合学院妇科教研室（石家庄050091）	duhuilan@ 163.com

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中文摘要:

目的研究补肾调经方对人卵泡壁颗粒细胞Smad1（drosophila mothers against decapentaplegic protein 1）、Smad5、 Smad8及 Smad4表达的影响。方法将66例接受体外受精－胚胎移植（in vitro fertilization embryo transfer，IVF ET）不孕症患者按1∶2的比例分为中药加长方案控制性超排卵组（简称治疗组， 23例）和长方案控制性超排卵组（简称对照组， 43例）。应用实时荧光定量PCR（real time PCR）法测定取卵日成熟卵泡壁颗粒细胞Smad1、Smad5、Smad8、 Smad4 mRNA的表达；应用细胞免疫荧光方法观察两组颗粒细胞Smad1、Smad5、 Smad8和 Smad4蛋白的表达。结果治疗组颗粒细胞Smad1 mRNA及蛋白表达显著高于对照组（P<0.05）；两组Smad5、Smad8和 Smad4 mRNA及蛋白表达差异无统计学意义。结论补肾调经方提高妊娠率、改善卵巢功能的机制可能与上调壁颗粒细胞Smad1 mRNA和蛋白的表达有关。

英文摘要:

Objective To explore the effect of Bushen Tiaojing Recipe （BTR） on the expressions of drosophila mothers against decapentaplegic protein （Smad1）， Smad5， Smad8， and Smad4 on human mural granulosa cells. Methods Sixty six patients undergoing in vitro fertilization embryo transfer （IVF ET） were randomly assigned to two groups in the ratio of 1∶2， the treatment group and the control group. Twenty three patients in the treatment group were treated with BTR and GnRHa/FSH/hCG， while forty three patients in the control group were treated with GnRHa/FSH/hCG. The mRNA expressions of Smad1， Smad5， Smad8， and Smad4 on mural granulosa cells of the mature follicle were detected by real time PCR on the ovum retrieval day. The expressions of Smad1， Smad5， Smad8， and Smad4 at the protein level were observed using cell immunofluorescence method. Results The mRNA and protein expressions of Smad1 in the granulosa cells were significantly higher in the treatment group than in the control group （P<0.05）. There was no statistical difference in the mRNA and protein expressions of Smad5， Smad8， and Smad4 between the two groups. Conclusion The mechanisms of BTR for improving the pregnancy rate and the ovarian functions might be correlated with up regulating mRNA and protein expressions of Smad1 of human mural granulosa cells.

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