丹蛭降糖胶囊联合运动对糖尿病大鼠胰腺NADPH氧化酶亚单位p22phox表达水平的影响

作者	单位	E-mail
吴元洁,郑书国,吴元波
方朝晖	安徽中医学院第一附属医院内分泌科（合肥 230031）	fangzhaohui@medmail.com.cn

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中文摘要:

目的探讨益气养阴活血方丹蛭降糖胶囊联合运动对糖尿病大鼠胰腺烟酰胺腺嘌呤二核苷酸磷酸（nicotinamide adenine dinucleotide phosphate， NADPH）氧化酶亚单位22 kD多肽（p22phox）蛋白表达水平的影响。方法雄性Wistar大鼠60只，分为正常组、模型组、运动组、丹蛭降糖胶囊组（简称中药组）及丹蛭降糖胶囊加运动组（简称中药加运动组），用小剂量链脲佐菌素（STZ）联合高脂饲料的方法建立糖尿病大鼠模型，用免疫组化法检测大鼠胰腺组织中p22phox、8-羟基脱氧鸟苷（8-hydroxy-2-de-axyguanine， 8-OHdG）的表达，用Western blot检测各组p22phox表达水平。结果模型组p22phox、8-OHdG的表达水平明显高于正常组，差异有统计学意义（P<0.01），免疫组化观察到p22phox、8-OHdG主要在胰岛细胞胞浆中表达，中药、运动能明显降低p22phox、8-OHdG水平（P<0.01），运动与中药联用对降低p22phox、8-OHdG水平有协同作用（P<0.05）。结论运动、丹蛭降糖胶囊及两者联用能降低NADPH氧化酶的表达，减少氧化应激的来源，从而保护胰岛β细胞。

英文摘要:

Objective To explore effects of exercise combined Danzhi Jiangtang Capsule （DJC） on the protein expression of nicotinamide adenine dinucleotide phosphate （NADPH） oxidase p22phox in pancreatic tissues of diabetic rats. Methods Sixty male Wistar rats were injected with low dose of streptozotocin and fed with high fat diet to establish a diabetic rat model. The levels of p22phox and 8-hydroxy-2-de-axyguanine （8-OHdG） protein in pancreatic tissues were detected by immunohistochemical method， and the level of p22phox protein was also detected by Western blot in the normal group， the model group， the excise group， the DJC group， and the DJC+excise group， respectively. Results The expression levels of p22phox and 8-OHdG protein in pancreatic tissues were significantly higher in the model group than in the normal group （P<0.01）. p22phox and 8-OHdG were mainly expressed in the cytoplasm of pancreatic cells. After administration of exercise or DJC， the expression levels of p22phox or 8-OHdG protein in pancreatic tissues decreased significantly （P<0.01）. Exercise combined DJC had synergistic effects in decreasing expressions of p22phox and 8-OHdG （P<0.05）. Conclusion Exercise， DJC， and exercise combined DJC could protect islet β cells by decreasing the expression of NADPH oxidase in β cells and reducing sources of oxidative stress.

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