鳖甲煎丸抑制肝癌细胞增殖、黏附及侵袭作用的实验研究

目的研究鳖甲煎丸对肝癌细胞生长增殖、黏附及侵袭能力的影响，初步探讨鳖甲煎丸抗肝癌细胞的转移、侵袭作用机制。方法运用血清药理学方法，以高、中剂量的鳖甲煎丸含药血清和空白血清分别培养肝癌细胞HepG2，采用MTT 比色法、细胞黏附实验和transwell侵袭实验检测药物对细胞生长、黏附和侵袭作用的影响，以进一步阐明鳖甲煎丸抗肝癌细胞的转移、侵袭作用机制。结果高剂量和中剂量含药血清可显著抑制肝癌细胞生长增殖，对基底膜的黏附及侵袭穿越迁移亦有抑制作用，且该作用与药物浓度有关。结论鳖甲煎丸能显著抑制肝癌细胞的生长增殖、黏附及转移侵袭。

英文摘要:

Objective To investigate effects of Biejiajian Pill （BP） on the proliferation， adhesion， and invasion of hepatoma carcinoma cells （HepG2）， and to primarily explore the mechanisms for fighting against metastasis and invasion. Methods Using seropharmacological methods， HepG2 cells were respectively cultured by high and middle dose BP containing serums and the vehicle serum. Using MTT colorimetry， cell adhesion test， and Transwell invasion test， effects of BP on the proliferation， adhesion， and invasion of HepG2 cells were detected， thus further exploring the mechanisms for fighting against the metastasis and invasion of HepG2 cells. Results High and middle dose BP containing serums could significantly prohibit the growth and proliferation， the adhesion and invasion of HepG2 cells on the basilar membrane. Besides， these effects were correlated with the concentrations of BP. Conclusion BP could effectively inhibit the growth and proliferation， adhesion and invasion of HepG2 cells.

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