心复力颗粒对阿霉素致扩张型心肌病心力衰竭大鼠心肌细胞凋亡的影响

作者	单位	E-mail
沈启明,王少霞,李杨,张瑞华
马丽红	中国医学科学院北京协和医学院阜外心血管病医院中医科（北京100037）	mlh4463@163.com

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中文摘要:

目的探讨心复力颗粒对阿霉素（adriamycin，ADR）致扩张型心肌病（dilated cardiomyopathy，DCM）心力衰竭大鼠心肌细胞凋亡的影响。方法72只SD大鼠随机分为正常组，模型组，厄贝沙坦组，心复力颗粒低、中、高剂量组，除正常组外均予ADR腹腔注射制备DCM心衰大鼠模型，正常组注射等量生理盐水，每周1次，持续6周。从注射ADR第5周各组开始灌胃给药，将心复力颗粒用蒸馏水配成0.5 g/mL的混悬液，心复力颗粒低、中、高剂量组的灌胃剂量分别为0.675、1.350、2.700 g/（kg·d），厄贝沙坦组剂量为50 mg/（kg·d），正常组和模型组给予与中剂量组等量生理盐水，每天上午灌胃1次，连续给药4周。用原位末端转移酶标记（terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling，TUNEL）技术法检测大鼠心肌细胞凋亡，免疫组化方法检测心肌细胞Bcl-2、Bax蛋白表达。结果与正常组比较，模型组心肌细胞凋亡率和Bax蛋白表达明显升高，Bcl-2蛋白表达水平及Bcl-2/Bax比值明显下降（P<0.05）。与模型组比较，心复力颗粒高剂量组和厄贝沙坦组Bcl-2表达水平及 Bcl-2／Bax比值显著升高（P<0.01）；心复力颗粒各剂量组和厄贝沙坦组Bax蛋白表达水平明显下降（P<0.01）。结论心复力颗粒能明显减轻DCM心衰大鼠心肌细胞凋亡，逆转心脏重构的发生、发展，其机制可能与调控Bcl-2、Bax蛋白表达有关。

英文摘要:

ObjectiveTo investigate the effects of Xinfuli Granule （XG） on cardiomyocyte apoptosis in rats with adriamycin-induced dilated cardiomyopathy （DCM）. MethodsSeventy-two male SD rats were randomly divided into 6 groups， i.e.， the normal control group， the model group， the irbesartan group， the low dose XG group， the medium dose XG group， and the high dose XG group. The DCM heart failure rat model was established using peritoneal injection of ADR. Equal volume of normal saline was injected to those in the normal control group， once per week for 6 consecutive weeks. The medication was started from the 5th week by gastrogavage. XG was dispensed into 0.5 g/mL suspension with distilled water. The XG was administered at the daily dose of 0.675 g/kg， 1.350 g/kg， and 2.700 g/kg to those in the low dose XG group， the medium dose XG group， and the high dose XG group， respectively. Irbesartan was administered to rats in the irbesartan group at the daily dose of 50 mg/kg. Equal volume of normal saline was administered to those in the normal control group and the model group by gastrogavage， once in the morning for 4 consecutive weeks. Myocardial apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL）， and the expressions of the Bcl-2 and Bax protein of cardiomyocytes were measured by immunohistochemical assay. ResultsCompared with the normal control group， the cardiomyocyte apoptosis rate and Bax expression level obviously increased， but the expression of Bcl-2 and the Bcl-2／Bax ratio decreased significantly in the model group （P<0.05）. Compared with the model group， the expression of Bax and the Bcl-2/Bax ratio increased significantly in the high dose XG group and the irbesartan group （P<0.01）. The Bax expression level obviously decreased in all groups except the normal control group （P<0.01）. ConclusionsXG could obviously attenuate cardiomyocyte apoptosis in the adriamycin-induced DCM rats， and reverse the occurrence and development of heart reconstruction. The underlying mechanism might be related to regulating and controlling the expressions of Bax and BcI-2.

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