社交应激动物模型的建立及其效果评价

目的建立一种用于模拟人类心理疾病的社交应激动物模型，为深入开展抑郁症的实验研究打下基础。方法取8只C57BL/6J小鼠（简称“C57小鼠”）作为应激组，连续给予社交失败应激刺激10天，另取8只C57小鼠作为正常对照组，不接受应激，后用Noldus动物行为学视频跟踪系统对C57小鼠进行行为学分析，主要包括两组小鼠在有或无攻击性CD 1小鼠存在时，在社交失败应激旷场中的运动轨迹及两组小鼠在社交应激失败旷场首次出现在社交区的时间、在社交区的持续停留时间和首次进入角落区的时间及在角落区中的持续停留时间，通过这些数据来分析C57小鼠行为学的变化，从而推断出C57小鼠的心理变化。结果与正常对照组比较，应激组C57小鼠的行为学有明显改变，表现为在社交区域的轨迹明显减少，其运动轨迹主要分布在角落区及其周围较小范围内的区域，且在社交区停留时间缩短、首次出现在社交区的时间延长（P<0.05），在角落区停留时间延长、首次出现在角落区的时间缩短（P<0.05）。结论通过社交失败应激可以建立一种与人类抑郁症相符的抑郁样动物模型。

英文摘要:

ObjectiveTo establish a social defeat stress model for simulating the human mental disease， thus laying a foundation for in depth laboratory research on depression. MethodsEight C57BL/6J mice （abbreviated as C57 mice） were recruited as the stress group. They were subject to psychological stress of social defeat for 10 successive days. Besides， another 8 C57 mice were selected as the normal control group （receiving no stress）. The Noldus Ethovision was used to evaluate the depressive behavior of mice. The date was acquired in the case of with or without aggressive CD 1 mice in the social defeat open field （SDOF）， and it included the two groups of mice′s trajectory in the SDOF and the first time of the two groups of mice′s entry into the interactive area of the SDOF， the residence time of the two groups of mice in the interactive area of the SDOF， the first time of the two groups of mice′s entry into the corner areas of the SDOF and the residence time of the two groups of mice in the corner areas of the SDOF. All data were used to analyze the changes in the behavior of the C57 mice， thus inferring the psychological changes of C57 mice. ResultsThe mice in the social stress group showed significant behavioral differences when compared with the normal control group. Their trajectories in the interactive area of the SDOF were significantly reduced. The trajectories of the mice in the social stress group were mainly distributed in the corner areas of the SDOF and its surrounding area within the smaller range. The residence time of mice in the social stress group in the interactive area of the SDOF was shortened （P<0.05）. The first time for the mice in the social stress group to enter the interactive area of the SDOF was extended （P<0.05）. Their residence time in the corner areas of the SDOF was shortened （P<0.05）. The first time for mice in the social stress group to enter the corner areas of the SDOF was extended （P<0.05）. ConclusionAn animal model of depressive behavior can be established by social defeat stress， which was consistent with human depression.

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