赤芍总苷对D-半乳糖诱导衰老大鼠脑组织
TOLL受体和IL-33的实验研究

Effects of Total Paeony Glucosides on mRNA Expressions of Toll Receptors and Interleukin 33 in the Brain Tissue of D galactose Induced Aging Rats： an Experimental Research

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DOI：10.7661/CJIM.2013.06.0830

中文关键词: 赤芍总苷衰老大鼠 TOLL受体白细胞介素-33

英文关键词:total paeony glycoside aging rat TOLL receptor interleukin-33

基金项目:黑龙江省教育厅科学技术研究资助项目（No12521652）

作者	单位	E-mail
张海燕,刘忠锦
陈志伟	齐齐哈尔医学院组织学与胚胎学教研室（黑龙江齐齐哈尔161006）	czwzp@sina.com

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中文摘要:

目的观察赤芍总苷（total paeony glucosides， TPGs）对D-半乳糖（D-galactopyranos， D-gal）诱导衰老大鼠脑组织TOLL受体（Toll like receptors，TLR 4）mRNA和白细胞介素-33 （interleukin-33，IL-33）mRNA的影响。方法50只SD大鼠随机分为5组，即空白对照组，模型组，TPG高、中、低剂量组，每组10只。空白对照组给予皮下注射等容量生理盐水，其余40只均皮下注射10%D-gal 0125 mL/g，每天 1 次，连续8 周，诱导衰老大鼠模型。从制备模型的第5 周开始，对TPG高、中、低剂量组大鼠腹腔注射D-gal同时，分别按300、150 和 75 mg/kg的剂量给予TPG灌胃，每天 1次，连续4 周。模型组和空白对照组给予灌胃等容量的生理盐水，每天1 次。采用 Morris 水迷宫测定法检测学习记忆能力，RT-PCR检测大鼠脑组织中TLR4 mRNA和IL-33 mRNA变化。结果与空白对照组比较，模型组大鼠学习记忆能力下降，差异有统计学意义（P<0. 05）；与模型组比较，各给药组大鼠治疗后学习记忆能力明显增强，并且存在剂量依赖性，差异有统计学意义（P<0. 05）。与空白对照组比较，模型组大鼠TLR4 mRNA和IL-33 mRNA含量明显增多，差异有统计学意义（P<0. 05）；与模型组比较，各给药组大鼠用药后TLR4 mRNA和IL-33 mRNA明显下降，并且存在剂量依赖性，差异均有统计学意义（P<005）。结论TPG通过调节TLR4 mRNA和IL-33 mRNA的变化，改善 D-gal 诱导衰老大鼠学习记忆能力。

英文摘要:

ObjectiveTo investigate effects of total paeony glucosides （TPGs） on the expressions of Toll receptors （TLR4） and interleukin-33 （IL-33） in the brain tissue of D-galactose-induced aging rats. MethodsFifty SD rats were randomly divided into 5 groups， i.e.， the blank control group， the model group， the high dose TPG group， the middle dose TPG group， and the low dose TPG group， 10 in each group. Equal volume of normal saline was subcutaneously injected to rats in the blank control group， while 10% D-galactose was subcutaneously injected to rats in the rest groups at 0.125 mL/g， once a day for 8 successive weeks to induce the aging rat model. TPG was administered at 300 mg/kg， 150 mg/kg， and 75 mg/kg to rats in the high， middle， and low dose TPG groups while injecting D-galactose from the 5th week of model preparation， once daily for 4 successive weeks. Equal volume of normal saline was administered to rats in the blank control group and the model group， once daily. The capability for learning and memory was detected using Morris water. The mRNA expressions of TLR4 and IL-33 in the brain tissue were detected using ELISA. ResultsCompared with the blank control group， the capability for learning and memory decreased in the model group with statistical difference （P<0.05）. Compared with the model group， the capability for learning and memory was obviously improved in all the medicated groups in a dose-dependent manner， showing statistical difference （P<0.05）. Compared with the blank control group， mRNA expressions of TLR4 and IL-33 in the brain tissue obviously increased after medication in the model group， showing statistical difference （P<0.05） . Compared with the model group， mRNA expressions of TLR4 and IL-33 in the brain tissue obviously decreased after medication in all the medicated groups in a dose-dependent manner， showing statistical difference （P<0.05）. ConclusionTPGs improved D-galactose induced aging rats′ capability for learning and memory through regulating changes of TLR4 and IL-33 expressions.

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