黑蒜提取液对Lewis肺癌细胞株的放疗增敏作用

目的探讨黑蒜提取液（black garlic extract，BGE）对肺癌Lewis细胞放射敏感性的影响。方法采用MTT法测定BGE作用后肺癌Lewis细胞增殖率，平板集落形成实验观察BGE联合放疗对细胞克隆形成率的影响，Hoechst荧光染色观察细胞形态的变化，流式细胞仪检测BGE作用后细胞周期的变化，Real time-PCR检测B淋巴细胞瘤－2（bcl-2）、凋亡促进蛋白（bax）mRNA的表达。结果 BGE对肺癌Lewis细胞株生长有明显的抑制作用。BGE联合60Co γ射线照射能够促进Lewis细胞凋亡，诱导Lewis细胞的G2/M期阻滞，明显降低bcl-2表达，促进bax高表达。结论黑蒜提取液对肺癌Lewis细胞具有放射增敏作用，其增敏机制可能与其改变细胞周期及影响bcl-2、bax蛋白表达有关。

英文摘要:

Objective To explore the radiosensitization effect of black garlic extract （BGE） on lung cancer cell line Lewis cells. Methods The inhibition rate of lung cancer cells after BGE action was detected by MTT. Effect of BGE combined radiotherapy on the colony formation rate was observed by cloning formation assay. Changes of the cell morphology were observed by Hoechst staining. Changes of the cell cycle were detected by flow cytometry. Real time PCR was used to detect mRNA expressions of bcl-2 and bax. Results BGE could have significant inhibitory action on the growth of lung cancer Lewis cells. The combination of BGE and radiotherapy （by 60Co γ） significantly induced Lewis cells′ apoptosis in G2/M stage， obviously decreased the expression of bcl-2， and up-regulated the expression of bax. Conclusions BGE could sensitize the lung cancer Lewis cells to ionizing irradiation. This effect might be probably caused by changing the cell cycles and affecting expressions of bax and bcl-2.

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