丹莪妇康煎膏提高子宫内膜异位症体外受精－胚胎移植患者

目的研究丹莪妇康煎膏提高子宫内膜异位症体外受精－胚胎移植（IVF-ET）患者周期卵细胞质量和胚胎质量的作用机理。方法将70例腹腔镜确诊子宫内膜异位症行IVF-ET的患者随机分为两组，治疗组（35例）为丹莪妇康煎膏加控制性超促排卵组，对照组（35例）为安慰剂加控制性超促排卵组，另取35例因男方因素行卵细胞浆内单精子注射（ICSI-ET）者设为正常对照组。应用Western blot法测定取卵日成熟卵泡液颗粒细胞中生长分化因子－9（GDF-9）的含量；RT-PCR法检测颗粒细胞中GDF-9 mRNA的表达；并比较各组的获卵数、卵裂率、受精率、优质胚胎率及妊娠率等。结果颗粒细胞中GDF-9 mRNA的表达，治疗组显著高于对照组，差异有统计学意义（P<0.05），与正常对照组比较差异无统计学意义。颗粒细胞中GDF-9的表达治疗组高于对照组，但差异无统计学意义（P>0.05），与正常对照组比较差异亦无统计学意义。卵裂率治疗组与对照组比较差异无统计学意义（P>0.05）。受精率、优质胚胎率治疗组均高于对照组，差异有统计学意义（P<0.05）；与正常对照组比较差异无统计学意义。结论丹莪妇康煎膏有提高子宫内膜异位症IVF-ET患者卵细胞和胚胎质量的作用，其作用机制可能与调控颗粒细胞GDF-9 mRNA水平有关。

英文摘要:

Objective To study the mechanism of Dan′e Fukang Soft Extract （DFSE） on improving oocyte and embryo qualities in endometriosis patients undergoing in vitro〖QX）〗 fertilization-embryo transfer （IVF-ET）. Methods Totally 70 patients with endometriosis confirmed by laparoscope were randomly assigned to two groups，the treated group and the control group， 35 cases in each group. Patients in the treated group were treated with DFSE +controlled ovarian hypestimulation （COH）， while those in the control group were treated with DFSE placebo +COH. Besides， recruited were another 35 subjects undergoing intracytoplasmic sperm injection-embryo transfer （ICSI-ET） as a normal control group. The content of growth differentiation factor 9 （GDF-9） in the granulocytes of the mature follicular fluid on the oocyte retrieval day was determined by Western blot. The mRNA expression of GDF-9 was detected by RT-PCR. The oocyte retrieval number， the cleavage rate，the fertilization rate，the high-quality embryo rate， and the pregnancy rate were compared. Results The mRNA expression of GDF-9 in the granulocytes was significantly higher in the treated group than in the control group， showing statistical difference （P<0.05）， but with no statistical difference when compared with that of the normal control group. There was no statistical difference in the cleavage rate between the two groups （P>0.05）. The fertilization rate and the high-quality embryo rate were higher in the treated group than in the control group， showing statistical difference （P<0.05）， but with no statistical difference when compared with that of the normal control group. Conclusions DFSE could improve the oocyte and embryo qualities of endometriosis patients undergoing IVF-ET. Its mechanism might be associated with regulating the GDF-9 mRNA level of granulocytes.

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