苦参碱对MCF-7细胞Fas、VEGF及端粒酶活性的影响

目的研究苦参碱对人乳腺癌MCF-7细胞Fas、VEGF及端粒酶活性的影响。方法体外培养人乳腺癌MCF-7细胞，随机分组，实验组细胞加入苦参碱溶液，对照组或阴性对照组细胞加入等量培养液，阳性对照组细胞加入端粒酶抑制剂莪术油。采用倒置显微镜下观察细胞形态学变化；TRAP-ELISA法检测端粒酶活性；免疫细胞化学法检测MCF-7细胞中Fas、VEGF蛋白表达情况。结果苦参碱对乳腺癌细胞有明显的生长抑制作用和促凋亡作用；不同浓度苦参碱处理MCF-7细胞24、48、72 h后，端粒酶活性随苦参碱浓度增加和作用时间延长而逐渐下降，呈剂量—效应正相关和时间—效应正相关；苦参碱能够上调MCF-7细胞Fas蛋白表达和下调MCF-7细胞VEGF蛋白表达。结论苦参碱对MCF-7细胞具有明显的生长抑制作用和促凋亡作用，可能是通过上调Fas蛋白表达、抑制端粒酶活性诱导乳腺癌细胞凋亡及通过下调VEGF蛋白表达，抑制肿瘤血管形成等实现的。

英文摘要:

Objective To study the effect of matrine on Fas， VEGF， and activities of telomerase of MCF-7 cells. Methods In vitro cultured human breast cancer MCF-7 cells were randomly divided into the experimental group and the control group. The matrine solution was added in cells of the experimental group. Equal volume of culture medium was added in cells of the control group or the negative control group. Zedoary Turmeric Oil， the telomerase inhibitor was added in cells of the positive control group. Morphological changes were observed under an inverted microscope. The telomerase activity was detected by TRAP-ELISA. Expressions of Fas and VEGF protein were detected by immunocytochemical assay. Results Matrine obviously inhibited the growth and induced apoptosis of breast cancer cells. MCF-7 cells were treated by matrine of different concentrations at 24， 48， and 72 h， the telomerase activity gradually decreased along with increased matrine concentration and prolonged action time， showing dose-effect and time-effect positive relations. Matrine could up-regulate Fas protein expression and down-regulate VEGF protein expression of MCF-7 cells. Conclusion Matrine showed obvious effect in inhibiting the growth of MCF-7 cells and promoting the apoptosis， which might be achieved by up-regulating the expression of Fas protein， inhibiting telomerase activity induced apoptosis of breast cancer cells， down-regulating the expression of VEGF protein， and inhibiting the tumor vascular formation.

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