血府逐瘀汤含药血清体外诱导骨髓基质干细胞表达Desmin和α-actin的实验研究

目的观察血府逐瘀汤含药血清能否诱导骨髓基质干细胞（MSCs）分化为心肌样细胞，以寻找安全有效诱导MSCs向心肌细胞分化的诱导剂。方法采用血清药理学方法进行诱导，制备血府逐瘀汤大鼠含药血清，分离培养大鼠 MSCs，MTT法检测血清细胞毒性，选用生长良好的P3细胞进行实验，将实验细胞分为3组，即空白对照组、血府逐瘀汤大鼠含药血清诱导组、5－氮胞苷诱导组。免疫细胞化学染色法检测心肌结蛋白（Desmin），α型心肌肌动蛋白（α-actin）的表达情况。结果诱导前各组MSCs细胞Desmin和α-actin表达阴性，个别弱阳性；其弱阳性表达率各组比较差异无统计学意义（P>0.05）。诱导后空白对照组MSCs细胞Desmin和α-actin表达阴性，个别弱阳性；血府逐瘀汤含药血清组和5－氮胞苷体外培养诱导组，MSCs细胞Desmin和α-actin表达阳性，其阳性表达率与空白对照组比较差异均有统计学意义（P<0.05）。结论血府逐瘀汤含药血清具有体外诱导大鼠骨髓基质干细胞分化为心肌样细胞的趋势，其作用可能参与了Desmin和α-actin的表达。

英文摘要:

Objective To observe whether Xuefu Zhuyu Decoction （XZD） could induce the differentiation of mesenchymal stem cells （MSCs） into cardiac myoid cells， thus seeking for safe and effective inducers. Methods The serum pharmacological method was used to induce. XZD containing serum was prepared. MSCs were isolated and cultured. The serum cytotoxicity was detected by MTT. The third generation of favorably grown cells was selected in this experiment. Cells were divided into three groups， i.e.， the vehicle control group， the XZD containing serum induced group， and the 5-azacytidine induced group. Expressions of Desmin and α-actin were detected by immunocytochemical staining method. Results Before induction protein expressions of Desmin and α-actin were negative， and few was weakly positive. There was no statistical difference in the weak positive expression rate among the 3 groups （P>0.05）. After induction protein expressions of Desmin and α-actin were negative， and few was weakly positive in the vehicle control group. Protein expressions of Desmin and α-actin were positive in the XZC containing serum induced group and the 5-azacytidine induced group. There was statistical difference in the positive expression rate when compared with the vehicle control group （P>0.05）.Conclusions XZD played a role in in vitro〖QX）〗 inducing differentiation MSCs to cardiac myoid cells. It might participate in expressions of Desmin and α-actin.

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