电针“夹脊穴”对压力诱导腰椎间盘退变模型兔髓核细胞超微结构的影响

Effect of Electroacupuncture at "Jiaji" on Ultrastructure of Nucleus Pulposus Cells in Rabbit Models of Lumbar Disc Degeneration Induced by Pressure

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DOI：10.7661/j.cjim.20220905.311

中文关键词: 电针椎间盘退变髓核细胞超微结构兔

英文关键词:electroacupuncture intervertebral disc degeneration nucleus pulposus cell cell ultrastructure rabbit

基金项目:国家自然科学基金面上项目（No. 81774392；No. 82074517）

作者	单位
王昆秀,邹璟,汪敏,张艳琳,黄国付	1.湖北中医药大学针灸骨伤学院（武汉 430061）；2.湖北省武汉市第一医院针灸科（武汉 430000）；3.广东省深圳市龙岗区妇幼保健院中医科（广东 518172）

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中文摘要:

目的观察轴向压力诱导的椎间盘（IVD）退变（IDD）模型兔髓核细胞形态结构的变化，探究电针对其变化的影响。方法将雄性新西兰大白兔随机分为正常组、模型组、假模型组、模型+电针组、模型+假电针组，每组5只。采用造模器对L4~5 IVD施加轴向压力复制IDD模型，假模型组仅安装造模器而不施加压力。造模成功后，模型+电针组电针L4、L5双侧“夹脊穴”；模型+假电针组仅针刺，无电针；正常组、模型组、假模型组仅捆绑，不予其他干预，各组干预均1次/天，20 min/次，连续6 天为1个疗程，共4个疗程。观察并记录各组兔的一般情况、行走疼痛评分和综合反应情况评分，采用核磁共振成像（MRI）比较各组IVD的信号强度，采用HE染色和透射电镜观察各组IVD髓核组织形态及亚细胞结构。结果正常组一般情况良好，未出现行走疼痛现象，IVD信号呈均匀的高亮影，髓核组织形态正常，髓核细胞线粒体结构清晰完整，假模型组上述情况基本类似于正常组；与正常组比较，模型组一般情况不佳，行走疼痛评分明显升高（P<0.01），综合反应情况评分显著降低（P<0.01），IVD信号消失，髓核组织破坏，髓核细胞数量减少、线粒体结构缺失，胶原纤维沉积；与模型组比较，模型+电针组上述现象不同程度好转，行走疼痛评分降低（P<0.05），综合反应情况评分升高（P<0.05），模型+假电针组改善不明显。结论轴向压力可显著破坏髓核组织及细胞形态结构，电针能够不同程度地改善其诱导的退变IVD髓核细胞线粒体超微的改变，延缓IDD。

英文摘要:

Objective To observe the morphological and structural changes of nucleus pulposus cells induced by axial pressure in intervertebral disc （IVD）degeneration （IDD） model rabbits， and to explore the effects of electroacupuncture （EA） on these changes. Methods Male New Zealand rabbits were randomly divided into the following groups：normal control， model， sham， model+EA， and model+sham EA， 5 rabbits in each group. In order to replicate the IDD model， longitudinal pressure was applied to the L4~5 IVD of rabbits using a modeling device. The sham model group was only equipped with a modeling device but no pressure. In order to replicate the IDD model， the L4~5 IVD of the rabbits were subjected to longitudinal pressure by the modeling device， and the sham group was only installed with the modeling device but without pressure. After successful modeling， EA was applied to the L4 and L5 "Jiaji" of the model+EA group. The model+sham EA group was only given acupuncture without EA. The normal control group， model group， and sham group were only bundled without other interventions. Each group was intervened for 4 courses， and their intervention was applied for 20 min each time， once a day. Continuous treatment of 6 days was a course of treatment， 4 courses in total. The general condition， walking pain score， and comprehensive reactions of each group of rabbits were observed and recorded. The signal intensities of the IVD of the rabbits in each group were compared by magnetic resonance imaging （MRI） . The morphology and subcellular structure of the nucleus pulposus of the IVD were observed by HE staining and electron microscopy. Results The general condition of the normal group was favorable， and they walked without pain. The signal of the IVD was uniform， the shape of the nucleus pulposus tissue was normal， and the structure of the nucleus pulposus cells was clear and complete. The sham group was basically similar to the normal group. Compared with the normal group， the general condition of the rabbits in the model group was not favorable，walking pain score incresed（P<0.01）， overall response scores decreased （P<0.01），with disappeared IVD disc signals， destructed nucleus pulposus tissues， reduced number of nucleus pulposus cells， deposited collagen fibers， and lost mitochondrial structure in nucleus pulposus cells. Compared with the model group， the above phenomena in the model+EA group were improved to various degrees， and walking pain score decreased （P<0.05）， overall response scores increased （P<0.05）. But these symptoms were not significantly improved in the model+sham EA group. Conclusions Long-term longitudinal pressure damaged the nucleus pulposus tissue and cell morphological structure of the IVD significantly. EA reversed the pressure-induced morphological changes of the nucleus pulposus cells of the IVD and postponed IDD.

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