益心化浊方对慢性心力衰竭大鼠肠道菌群结构与功能的影响

作者	单位
陈婵;赵博文;吕铮天;李心怡;娄洋;毛威	1.浙江中医药大学附属江南医院杭州市萧山区中医院中医内科（杭州 311201）,2.浙江中医药大学附属第一医院心内科（杭州 310006）,3.浙江省中西医结合循环系疾病诊治重点实验室（杭州 310006）

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中文摘要:

目的探讨益心化浊方对慢性心力衰竭大鼠肠道菌群结构与功能的作用。方法采用左冠状动脉结扎术建立慢性心力衰竭模型，将造模成功的14只大鼠随机分为模型、益心化浊方组，每组7只，另设7只SD大鼠为假手术组，只穿线不结扎。益心化浊方组给予益心化浊方2.40 mg/mL灌胃，假手术组和模型组动物给予等量生理盐水灌胃，干预4周。采用16S rRNA基因测序技术测定大鼠肠道细菌，应用Alpha、LEfSe、PLS-DA对数据进行分析，并将基因序列在KEGG功能谱数据库中进行功能预测。结果与模型组比较，益心化浊方组乳杆菌目、气球菌科、乳酸菌科、草酸杆菌科、假单胞菌科、假单胞菌属、贪铜菌属、沙雷氏菌属、费克蓝姆氏菌属上调（P<0.05），梭杆菌门、梭杆菌纲、腐生螺旋菌纲、梭杆菌目、腐螺旋菌目、噬几丁质杆菌属、克里斯滕森菌科、明串珠菌科、孪生菌科、沉积物杆状菌属、02d06菌、拟无枝酸菌下调（P<0.05）。益心化浊方组富集于分节丝状菌属。在代谢层面上益心化浊方组与异源生物降解和代谢、核苷酸代谢、萜类和聚酮类代谢、氨基酸代谢、辅因子和维生素代谢、脂质代谢、聚糖生物合成和代谢关联；在细胞功能层面上与运输和分解、细胞生长和死亡密切关联；在生物系统层面上与神经系统、排泄系统、内分泌系统及循环系统相关。结论益心化浊方具有促进慢性心力衰竭大鼠肠道正常菌群增殖、改善肠道功能的调控作用。

英文摘要:

Objective To explore the effect of Yixin Huazhuo Formula（YXHZF） on the structure and function of intestinal flora in rats with chronic heart failure. Methods The chronic heart failure rat model was established by left coronary artery ligation. Totally 14 SD rats with chronic heart failure were randomly divided into model group and YXHZF group，7 rats in each group. Another 7 SD rats in sham group were thread only without ligation. The rats in YXHZF group were intragastric administration 2.40 mg/mL YXHZF，the rats in sham and model group were intragastric administration same amount of normal saline，the intervention time was 4 weeks. The16S rRNA gene sequencing technology was used to determine the intestinal bacteria of rats. The data was analyzed by Alpha，Lefse and PLS-DA. Gene sequence was predicted in KEGG functional spectrum database. Results Compared with the model group，Lactobacillales，Aerococcaceae，Lactobacillaceae，Oxalobacteraceae，Pseudomonadaceae，Pseudomonas，Cupriavidus，Serratia，and Facklamia increased（P<0.05），Fusobacteria，Fusobacteriia，Saprospirae，Fusobacteriales，Saprospirales，Chitinophagaceae，Christensenellaceae，Leuconostocaceae，Gemellaceae，Sediminibacterium，02d06，and Amycolatopsis decreased in YXHZF group（P<0.05）. YXHZF group was enriched in Candidatus Arthromitus. At the metabolic level，YXHZ group was significantly associated with metabolism of xenobiotics biodegradation，nucleotide，terpenoids，polyketides，amino acids，cotactors，vitamins，lipid，and glycan biosynthesis. At the cell function level，YXHZF group was significantly associated with transportation，decomposition，cell growth and death. At the level of biological system，YXHZ group was significantly associated with nervous system，excretory system，endocrine system and circulatory system. Conclusions YXHZF can promote the proliferation of normal intestinal flora and improve intestinal function in rats with chronic heart failure.

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