| 李映欧,高凤辉,张金妹,刘志云,张京,涂新义,刘建勋,李连达.野菊花提取物CI-2对体外培养乳鼠心肌细胞缺氧缺糖性损伤的保护作用[J].,1981,(2):93-97,131,132,127 |
| 野菊花提取物CI-2对体外培养乳鼠心肌细胞缺氧缺糖性损伤的保护作用 |
| The Protective Effect of Chrysanthemum Indicum L Extract(CI-2)on Myocardial Injury in Cultured Neonatal Rat Heart Cells Deprived of Oxygen and Glucose |
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| 中文关键词: 缺氧缺糖 培养乳鼠心肌细胞 野菊花提取物 心肌细胞损伤 保护作用 培养基 细胞水平 体外培养心肌细胞 心脏血流动力学 正常对照组 |
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| 中文摘要: |
| 本室在筛选中草药过程中发现野菊花对实验性犬心肌缺血有明显的保护作用,并进一步证实野菊花提取物 CI-2可减小心肌梗塞范围、减轻心肌损伤程度,且具有调整心脏血流动力学等作用。迄今国内外对野菊花防治冠心病作用的研究尚少报道,我室初步实验结果表明,它有可能成为一种新的防治冠心病有效的药物。因此有必要对野菊花及其有效成分作进一步的研究。为在活的细胞水平研究药物对心肌细胞的直接保 |
| 英文摘要: |
| An in vitro model of myocardial cell injury was established with primary monolayer cultures of neonatal rat heart cells by depriving the cultures of oxygen and glucose for 6 hrs.This new experimental model could imitate myocardial ischemia,for oxygen and substrate deprivation was the two important aspects of ischemia.Cellular injury caused by hypoxia and glucose deprivation resulted in ultrastructural changes,decrease in beating activity and significant leakage of lectate dehydrogenase（LDH）from the cells into the culture medium.LDH in medium, which reflected the degree of cellular damage,was used to evaluate protective effects of various drugs.The authors observed that CI-2 reduced the release of LDH from injured heart cells deprived of oxygen and glucose. The results indicated that CI-2 had direct effects in protecting the injured heart cells in cellular level.In addition, it was also observed that 10-5M propranolol showed similar protective effect while 10-4M isoprenaline exacerbated heart cell injury.And 10-4M dexamethasone was found to have no effect in protecting cellular injury in our experiments. |
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