| 牛建昭,贲长恩,王德福.气血注射液对氢化可的松所致大鼠肝损伤保护作用的研究——肝细胞超微结构立体计量分析[J].,1984,(10):618-621,581 |
| 气血注射液对氢化可的松所致大鼠肝损伤保护作用的研究——肝细胞超微结构立体计量分析 |
| The Protective Effect of Qi-Xue Injection on Rat Liver Damage Induced by Cortisone Acetate——Analysis of Stereometrical Quantitation of the Ultrastructure of Rat Liver Parenchymal Cells |
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| 中文关键词: 氢化可的松 大鼠肝损伤 肝细胞超微结构 注射液 计量分析 线粒体 立体计量 数密度 正常组 溶酶体 |
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| 中文摘要: |
| 实验用立体计量分析方法观察肝细胞超微结构,结果证实:气血注射液对氢化可的松所致大鼠肝损伤有一定保护作用,使肝细胞内线粒体、粗面内质网、微体、溶酶体的立体形态及数量维持正常。说明立体计量分析法比一般形态学研究更客观、更精确。 |
| 英文摘要: |
| 35 male rats,weighing 170-210g,were divided into three groups:(1)The experimental group(rats given orally a daily Qi-Xue injection 10ml/kg body weight+cortisone acetate 50mg/kg body weight for 6-8 days). (2)The cortisone group(cortisone acetate 50mg/kg body weight for 6 days).(3)The control group(normal saline for 8 days).Stereometrical analysis of ultrastructural changes of the hepatic parcnchymal cell was made with electron microscopy to study the protective effect of Qi-Xue injection on cortisone-induced liver damage. In the cortisone group,the average mitochondrial volume of parenchymal cell increased from 0.71μm~3 to 2.10μm~3(P<0.001)and the mitochondrial number per cell decreased to 43.1% that of the control group. In the experimental group,the average mitochondrial volume was 1.37μm~3.and the number of mitochondria per cell rose to 61.3% that of the control group(P<0.01).The ratio between surface area and volume of mitochondria of the control group was 6.58,it reduced to 4.55 in the cortisone group and rose to 5.59 in the experimental group.The volume fractions of mitochondria,rough endoplastic reticulum,peroxisome and lysosome of rats in the control group were 27.50%.17.78%.2.22% and 0.62% respectively.In cortisone group, the number and the volume fractions of rough endoplastic reticulum and peroxisome reduced,while the number of lysosome increased.The total volume of rough endoplastic reticulum in the experimental group increased by 44.4% that of the cortisone group(P<0.01).Through these experimems,it is found that Qi- Xue injection has protective effect on cortisone-induced rat liver damage. |
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