| 袁卫龙,陈灏珠,杨英珍,杨学义,林佑善,金佩英,周泰生.黄芪对培养大鼠心肌细胞感染Coxsackie B-2病毒的电生理研究[J].,1989,(6):355-357,326 |
| 黄芪对培养大鼠心肌细胞感染Coxsackie B-2病毒的电生理研究 |
| Effect of Astragalus membranaceus on Electrical Activities of Coxsackie B-2 Virus-Infected Rat Beating Myocardial Cells in Culture |
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| 中文关键词: 心肌细胞 细胞感染 大鼠心肌 搏动频率 病毒感染 细胞病变 原代单层培养 不同时间 正常对照组 细胞电活动 |
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| 中文摘要: |
| 本文报道应用常规细胞内微电极技术记录原代单层培养大鼠搏动心肌细胞感染 Coxsackie B-2病毒后不同时间的电生理参数变化和同时期在倒置相差显微镜下观察的心肌细胞搏动频率、节律、搏动%及细胞病变。在此基础上,研究黄芪对 Coxsackie B-2病毒感染体外培养搏动大鼠心肌细胞电活动的影响。结果提示从病毒感染后24～96h,黄芪对心肌细胞搏动频率、节律、搏动%、细胞病变和各项电生理参数等均有明显保护作用,心肌细胞的结构和功能均维持在大致正常状态。本文结果可为黄芪防治病毒性心肌炎提供新的线索。 |
| 英文摘要: |
| Beating Myocardial cell cultures of neonatal rats were prepared in vitro and infected with coxsackie B-2 virus.The cells were evaluated in the post-infected period for changes in beating percentage and cytopathic effect(CPE),alterations in the electrical activities by standard mi- croelectrode techniques,and the protective effect of Astragalus membranaceus(AM)on coxsackie B-2 virus-infected neonatal rat myocardial cell cultures was observed.The beating percentage began to decrease in the infected group at 24 hr and only 27.94±18.6% was beating at 96 hr after virus challenge,premature beats,tachycardia and fibrillation occurred commonly during the experiment. Meanwhile the CPE appeared rapidly from 1+～3+at the same interval.Resting potential,action potential amplitude,duration and rate of upstrake were shown a significant decrease through 24～96 hr(P<0.01).In contrast,the beating and electrical activities were nearly normal and less CPE was shown in myocardial cells treated with AM 1 hr after virus challenge through 24～96 hr(P<0.05).The results suggest that AM may be valuable in prophylaxis and treatment of acute coxsackie B-2 virus caused myocarditis. |
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