| 彭天庆,杨英珍.黄芪对柯萨奇B3病毒核糖核酸作用的研究及其机理探讨[J].,1994,(11):664-666 |
| 黄芪对柯萨奇B3病毒核糖核酸作用的研究及其机理探讨 |
| Effect and Mechanism of Astragalus Membranaceus on Coxsackie B3 Virus RNA in Mice |
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| DOI： |
| 中文关键词: 核酸链特异性原位杂交 柯萨奇B3病毒核酸 β－干扰素 病毒性心肌炎 黄芪 |
| 英文关键词:RNA-RNA in situ hybridization Coxsackie B3 Virus ribonucleic acid β-interferon myocarditis Astragalus membranaceus |
| 基金项目:上海市科委资助 |
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| 中文摘要: |
| 以体外转录合成、同位素３５Ｓ标记的肠道病毒特异负股ＲＮＡ为探针，对急性柯萨奇Ｂ３病毒（ＣＶＢ３）心肌炎小鼠心肌中的ＣＶＢ３－ＲＮＡ进行ＲＮＡ－ＲＮＡ原位杂交检测，利用图像分析仪对阳性杂交信号进行图像处理与定量分析；观察黄芪对ＣＶＢ３－ＲＮＡ含量的影响；同时对黄芪的抗病毒机理及其与β－干扰素的关系进行了初步探讨。结果发现在病毒感染的心肌组织中，黄芪组心肌坏死面积明显小于生理盐水对照组。黄芪对ＣＶＢ３－ＲＮＡ的复制有良好的抑制作用，其机制与β－干扰素（β－ＩＦＮ）无关。 |
| 英文摘要: |
| Using Balb/c mice infected with Coxsackie virus B3(CVB3)as a model,the effect of Astragalus membranaceus(AM)on CVB3-RNA has been observed in myocardial tissues of mice by RNA-RNA in situ hybridization with negative-strand RNA probes leballing with 35S and quantitative imaging analysis of positive hybridization signals.The mechanism of its effect on CVB3-RNA has also been investigated by induction with AM and detection of beta-interferon(β-IFN).Results showed that the copy numbers of CVB3-RNA as well as the histologic necrotic sizes in myocardial tissues of AM treated infected mice were significantly smaller than that in infected normal saline treated mice(P<0 .01,P<0.05) respectively,suggesting that AM could inhibit the replication of CVB3-RNA,but its effect on CVB3-RNA was not correlated with induction of β-IFN. |
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