孙林,易著文,虞佩兰.川芎嗪对人胎肾小球系膜细胞增殖的影响及其机理探讨[J].,1995,(3):134-136 |
川芎嗪对人胎肾小球系膜细胞增殖的影响及其机理探讨 |
Effect of Tetramethylhyrazine on Proliferation of Human Fetal Mesangial Cells |
|
DOI: |
中文关键词: 系膜细胞 增殖 川芎嗪 |
英文关键词:mesangial cell proliferation tetramethylhyrazine |
基金项目: |
|
Hits: 1400 |
Download times: 1297 |
中文摘要: |
在人胎肾小球系膜细胞(mesangialcell,MC)培养体系中加入川芎嗪后能抑制MC生长数量。川芎嗪0.6mmol、1.2mmol加入MC培养体系6天后,MC数量(活细胞个数/ml)分别为:37580±3475,27350±3418,明显低于对照组71850±5108(P<0.05);MC ̄3H-TdR摄取量(cpm)对照组为3575±306,加入川芎嗪0.6mmol、1.2mmol后分别为1017±201,583±271(P<0.01);加入川芎嗪1mmol于MC培养体系中,MC上清液(1:8稀释度)加入KD_(83)细胞体系中,KD_(83)细胞 ̄3H-TdR摄取量为2118±215,明显低于阴性对照组3890±295与阳性对照组4128±351(P<0.01)。提示川芎嗪抑制MC增殖的机理是通过降低MC自分泌IL-6而致。 |
英文摘要: |
Tetramethylhyrazine (TMP) 0. 6 and 1. 2 mmol were added to human fetal mesangial cell (MC)cultures for 6 days, and the amounts of MC (cells/ml) were 37580±3475 and 27350±3418 respectively,significantly lower than that 71850±5108 in control (P<0. 05) .The 3 ̄H-TdR incorporation by the MC with corresponding TMP were 1017±201 and 583±271, also significantly lower,than that 3575±306 in control (P<0. 01) . After adding the TMP (l mmol) to the cultures, the IL-6 bioactivity were 2118±215 that were markedly lower than those in controls (4128±351,P<0 . 01). It revealed that the TMP inhibited the growth of MC and the mechanism of its inhibition might be due to that TMP could reduce the IL-6. |
View Full Text View/Add Comment Download reader |